Selective digestion of Ba2+/Ca2+ alginate gel microdroplets for single-cell handling
Selective digestion of Ba2+/Ca2+ alginate gel microdroplets for single-cell handling
复制标题
DOI：
10.7567/jjap.57.06hh02
复制
发表时间：
2018-06-01
影响因子：
1.5
通讯作者：
Yasuda, Kenji
中科院分区：
文献类型：
--
作者：
Odaka, Masao;Hattori, Akihiro;Yasuda, Kenji
被聚合物微滴包裹的细胞是用于识别和分离单个细胞以进行基于单细胞的分析的有效平台。然而，一个关键的挑战是选择性地、非破坏性地和非侵入性地维持和释放微滴中捕获的细胞。我们开发了一种简单的方法，具有不同的消化特性的海藻酸盐微滴封装细胞。用溶胶状态的藻酸盐聚合物稀释细胞，并通过喷雾法将其包封到具有Ba 2+和Ca 2+的微滴中。当施加螯合缓冲液时，根据连接二价阳离子的螯合效率的差异消化海藻酸盐凝胶微滴;因此，形成两种类型的海藻酸盐微滴。此外，我们研究了海藻酸盐凝胶交换连接二价阳离子的能力，发现Ba-海藻酸盐微滴中的Ca 2+交换和Ca-海藻酸盐微滴中的Ba 2+交换都发生。这些结果表明，具有不同二价阳离子的藻酸盐微滴的混合物的潜在应用控制微滴的选择性消化，以提高单细胞的高通量、高含量的基于微滴的分离、分析或储存。(C)2018日本应用物理学会
Cells encapsuled by polymer microdroplets are an effective platform for the identification and separation of individual cells for single-cell-based analysis. However, a key challenge is to maintain and release the captured cells in the microdroplets selectively, nondestructively, and noninvasively. We developed a simple method of encapsulating cells in alginate microdroplets having different digestion characteristics. Cells were diluted with an alginate polymer of sol state and encapsulated into microdroplets with Ba2+ and Ca2+ by a spray method. When a chelating buffer was applied, alginate gel microdroplets were digested according to the difference in chelating efficiency of linkage-divalent cations; hence, two types of alginate microdroplets were formed. Moreover, we examined the capability of the alginate gel to exchange linkage-divalent cations and found that both Ca2+ exchange in Ba-alginate microdroplets and Ba2+ exchange in Ca-alginate microdroplets occurred. These results indicate that the potential applications of a mixture of alginate microdroplets with different divalent cations control the selective digestion of microdroplets to improve the high-throughput, high-content microdroplet-based separation, analysis, or storage of single cells. (C) 2018 The Japan Society of Applied Physics