A novel actin label: a fluorescent probe at glutamine-41 and its consequences.
A novel actin label: a fluorescent probe at glutamine-41 and its consequences.
复制标题
一种新型肌动蛋白标签：谷氨酰胺 41 的荧光探针及其后果。
DOI：
10.1021/bi00403a015
复制
发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Takashi,R
中科院分区：
文献类型：
--
作者：
Takashi,R
摘要：通过多肽分离和分析发现，在豚鼠肝脏谷氨酰胺转胺酶催化下，兔骨骼肌中丹基尸体碱[Ar-（5-氨基戊基）-5-（二甲氨基）萘-l-磺酰胺]的丹基荧光基团转移到肌动蛋白Gln-41上。作为时间的函数，标记度渐近于1mol丹酰/1mol肌动蛋白。在Gln-41上发现了约80-85%的附着丹酰荧光团。这种标记的g -肌动蛋白的聚合程度与对照肌动蛋白相同，但聚合速率大于对照肌动蛋白，临界浓度小于对照肌动蛋白。完全聚合伴随着附着荧光团的发射强度增加1.5-2.0倍。由此获得的标记f -肌动蛋白激活肌凝蛋白亚片段1
Revised Manuscript Received October 15, 1987 abstract: By peptide isolation and analysis, it has been shown that the dansyl fluorophore of dansylcadaverine [Ar-(5-aminopentyl)-5-(dimethylamino) naphthalene-l-sulfonamide] transfers to Gln-41 of actin from rabbit skeletal muscle when the reaction is catalyzed by guinea pig liver transglutaminase. As a function of time, thedegree of labeling asymptotically approaches 1 mol of dansyl/1mol of actin. About 80-85% of the attached dansyl fluorophore was found at Gln-41. Such labeled G-actin polymerizes to the same extent as control actin, but the polymerization rate is greater and the critical concentration is less than for control actin. Complete polymerization is accompanied by a 1.5-2.0-fold increase in the emission intensity of the attached fluorophore. Labeled F-actin thus obtained activates myosin subfragment 1