Systemic administration of α-lipoic acid suppresses excitability of nociceptive wide-dynamic range neurons in rat spinal trigeminal nucleus caudalis
Systemic administration of α-lipoic acid suppresses excitability of nociceptive wide-dynamic range neurons in rat spinal trigeminal nucleus caudalis
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DOI：
10.1016/j.neures.2018.06.003
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发表时间：
2019-07-01
影响因子：
2.9
通讯作者：
Takeda, M.
中科院分区：
文献类型：
--
作者：
Hidaka, S.;Kanai, Y.;Takeda, M.
尽管已报道α-硫辛酸 (LA) 对神经系统 T 型 Ca2+ 通道具有调节作用，但 LA 在体内的急性作用，特别是对三叉神经系统中伤害性传递的作用仍有待确定。本研究的目的是调查大鼠急性静脉注射 LA 是否会减弱宽动态范围 (WDR) 脊髓三叉神经尾核 (SpVc) 神经元对体内伤害性和非伤害性机械刺激的兴奋性。 17 个 SpVc 神经元对戊巴比妥麻醉大鼠的口面部机械刺激作出反应，进行细胞外单单位记录。本研究分析了对无害性和有害机械刺激的反应。 LA（1-100 mM，静脉注射）显着且剂量依赖性地抑制 SpVc WDR 神经元响应无害性和有害机械刺激的平均放电频率，并且在 5 分钟内观察到对无害性和有害机械刺激的放电频率的最大抑制。这些抑制作用持续大约10分钟。这些结果表明，急性静脉注射 LA 可能通过阻断 T 型 Ca2+ 通道来抑制三叉神经感觉传递，包括伤害感受。 LA可用作治疗三叉神经伤害性疼痛的治疗剂。 (C) 2018 作者。由 Elsevier B.V. 出版
Although a modulatory role has been reported for alpha-lipoic acid (LA) on T-type Ca2+ channels in the nervous system, the acute effects of LA in vivo, particularly on nociceptive transmission in the trigeminal system, remain to be determined. The aim of the present study was to investigate whether acute intravenous LA administration to rats attenuates the excitability of wide dynamic range (WDR) spinal trigeminal nucleus caudalis (SpVc) neurons in response to nociceptive and non-nociceptive mechanical stimulation in vivo. Extracellular single unit recordings were made from seventeen SpVc neurons in response to orofacial mechanical stimulation of pentobarbital-anesthetized rats. Responses to both non-noxious and noxious mechanical stimuli were analyzed in the present study. The mean firing frequency of SpVc WDR neurons in response to both non-noxious and noxious mechanical stimuli was significantly and dose-dependently inhibited by LA (1-100 mM, i.v.) and maximum inhibition of the discharge frequency of both non-noxious and noxious mechanical stimuli was seen within 5 min. These inhibitory effects lasted for approximately 10 min. These results suggest that acute intravenous LA administration suppresses trigeminal sensory transmission, including nociception, via possibly blocking T-type Ca2+ channels. LA may be used as a therapeutic agent for the treatment of trigeminal nociceptive pain. (C) 2018 The Authors. Published by Elsevier B.V.