Biophenols and Antioxidant Properties of Australian Canola Meal
Biophenols and Antioxidant Properties of Australian Canola Meal
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DOI：
10.1021/jf4026585
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发表时间：
2013-09-25
影响因子：
6.1
通讯作者：
Agboola, Samson
中科院分区：
文献类型：
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作者：
Obied, Hassan K.;Song, Yi;Agboola, Samson
在菜籽油的提取过程中，会产生大量的粗粉。从澳大利亚油菜籽粕（ACM）中提取生物酚，为低价值的农业工业副产品增加了价值。本研究探讨了生物酚含量和抗氧化活性的ACM，提取条件的影响，和品种差异。结果表明，Sinorphin是ACM中的主要生物酚。从粗提物和水解物中鉴定出31种化合物：2种二己糖苷，2种有机酸，4种硫代葡萄糖苷，17种芥子酸衍生物，2种环亚精胺生物碱，咖啡酸及其二己糖苷，山奈酚及其C-葡萄糖苷。ACM在DPPH中心点和ABTS（中心点+）实验中显示出明显的自由基清除活性。芥子油苷是ACM抗氧化活性的主要贡献者，而山奈酚芥子酰三葡萄糖苷异构体是最有效的抗氧化剂。生物酚含量在12.8和15.4毫克GAE/克DW之间。研究品种之间的差异一般是定量的。Tarcoola品种表现出最高的生物酚含量和抗氧化活性。
During the extraction of canola oil, large quantities of meal are produced. Extracting biophenols from Australian canola meal (ACM) adds value to an otherwise low-value agro-industrial byproduct. This study examined the biophenol content and the antioxidant activity of ACM, the impact of extraction conditions, and varietal differences. Sinapine was the principal biophenol in ACM. In crude and hydrolyzed extracts, 31 compounds were identified: 2 dihexosides, 2 organic acids, 4 glucosinolates, 17 sinapic acid derivatives, 2 cyclic spermidine alkaloids, caffeic acid and its dihexoside, kaempferol, and its C-glucoside. ACM showed significant free radical scavenging activity in DPPH center dot and ABTS(center dot+) assays. Sinapine was the chief contributor to ACM antioxidant activity, whereas kaempferol sinapoyl triglucoside isomer was the most potent antioxidant. Biophenol content ranged between 12.8 and 15.4 mg GAE/g DW. Differences among studied cultivars were generally quantitative. The Tarcoola cultivar showed the highest biophenol content and antioxidant activity.