Detection and characterization of human herpesvirus-8-infected cells in bone marrow biopsies of human immunodeficiency virus-positive patients
Detection and characterization of human herpesvirus-8-infected cells in bone marrow biopsies of human immunodeficiency virus-positive patients
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DOI：
10.1053/hupa.2001.22749
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发表时间：
2001-03-01
期刊：
影响因子：
3.3
通讯作者：
Brousset, P
中科院分区：
文献类型：
--
作者：
Meggetto, F;Cesarman, E;Brousset, P
我们研究了15例骨髓活检标本与人类免疫缺陷病毒感染的检测卡波西肉瘤疱疹病毒（KSHV/HHV-8）的DNA序列的一个非常敏感和特异性的聚合酶链反应（PCR）检测（3种不同的引物）。此外，我们使用抗病毒白细胞介素-6（VIL-6）和抗潜伏性核抗原-1（LNA-1）抗体的免疫组织化学定位组织切片上的感染细胞。在15例标本中，有6例（orf 26，orf 72，orf 75）PCR结果为阳性。有趣的是，在这6例Kaposi肉瘤患者中，有2例（均为Kaposi肉瘤）的单个核淋巴细胞中检测到vIL-6和LNA-1，而在骨髓基质细胞中未检测到vIL-6和LNA-1。在骨髓中检测到vIL-6阳性淋巴样细胞表明HHV-8感染的成分在该组织中归巢。vIL-6的局部释放可能在获得性免疫缺陷综合征骨髓中观察到的浆细胞增多中发挥了一定作用。《人文哲学》32：288-291。Copyright（C）2001 by W.B.桑德斯公司
We studied 15 bone marrow biopsy specimens from patients with human immunodeficiency virus infection for detection of Kaposi sarcoma herpesvirus (KSHV/HHV-8) DNA sequences by a very sensitive and specific polymerase chain reaction (PCR) assay (with 3 different sets of primers). In addition, we used immunohistochemistry with anti-viral interleukin-6 (VIL-6) and anti-latent nuclear antigen-1 (LNA-1) antibodies to localize the infected cells on tissue sections. Among the 15 samples, 6 had positive PCR results with the 3 sets of primers (orf26, orf72, orf75), Interestingly, in 2 of these 6 patients (both with Kaposi sarcoma) vIL-6 and LNA-1 were detected in mononuclear lymphoid cells but not in stromal cells of the bone marrow. The detection of vIL-6-positive lymphoid cells in bone marrow suggests a homing for HHV-8-infected elements in this: tissue. The local release of vIL-6 may play some role in the plasmacytosis observed in bone marrow in the acquired immunodeficiency syndrome. HUM PATHOL 32:288-291. Copyright (C) 2001 by W.B. Saunders Company.