Potential role of decidual apoptosis in the pathogenesis of miscarriages
Potential role of decidual apoptosis in the pathogenesis of miscarriages
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DOI：
10.3109/09513590.2011.633127
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发表时间：
2012-05-01
影响因子：
2
通讯作者：
Can, Alp
中科院分区：
文献类型：
--
作者：
Cinar, Ozgur;Kara, Fadil;Can, Alp
为研究正常妊娠和流产（自发性和复发性）蜕膜细胞凋亡的存在和分布，设计了正常对照（n = 12）和流产妊娠（n = 24）的比较免疫荧光组织标记。评估正常妊娠和流产中蜕膜细胞凋亡的存在和分布，凋亡细胞类型的表征和caspase依赖性通路的参与与TUNEL，抗活性caspase-3，抗泛细胞角蛋白和抗CD 45抗体进行了分析。正常的蜕膜表现出很少的凋亡细胞，而从复发性流产的蜕膜有一个显着较高数量的凋亡细胞优先定位于上皮下和动脉周围区域，在那里发生的蜕膜化。细胞凋亡通过半胱天冬酶依赖性途径发生。免疫细胞和上皮细胞均未被任何凋亡标志物染色阳性。凋亡细胞的数量增加，这是严格限制在动脉周围基质，特别是在复发性流产导致我们建议，蜕膜细胞凋亡可能会导致一系列的细胞功能障碍，可能会威胁到怀孕的过程。
To investigate the existence and the distribution of decidual apoptosis in normal pregnancies and miscarriages (spontaneous and recurrent), a comparative immunofluorescent tissue labelling of normal control (n = 12) and miscarried pregnancies (n = 24) was designed. Evaluation of the existence and distribution of decidual apoptosis in normal pregnancies and miscarriages, characterization of the apoptotic cell types and the involvement of caspase-dependent pathways was analyzed with TUNEL, anti-active caspase-3, anti-pancytokeratin and anti-CD45 antibodies. Normal decidua showed few apoptotic cells, whereas decidua from recurrent miscarriages had a significantly higher number of apoptotic cells preferentially localized to the subepithelial and periarteriolar regions, where the onset of decidualization occurs. Apoptosis occurred via a caspase-dependent pathway. Neither immune nor epithelial cells were positively stained for any apoptotic markers. The increased number of apoptotic cells, which are strictly restricted to the periarteriolar stroma particularly in recurrent miscarriages leads us to suggest that decidual apoptosis could result a series of cellular dysfunctions that may threaten the course of pregnancy.