S locus F-Box brothers:: Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear
S locus F-Box brothers:: Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear
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DOI：
10.1534/genetics.106.068858
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发表时间：
2007-04-01
期刊：
影响因子：
3.3
通讯作者：
Koba, Takato
中科院分区：
文献类型：
--
作者：
Sassa, Hidenori;Kakui, Hiroyuki;Koba, Takato
虽然最近的研究结果表明，在基于S- rase的配子体自交不亲和（GSI）系统中，F-box基因SFB/SLF控制着花粉部分S的特异性，但这些基因如何在该系统中起作用尚不清楚，并且花粉S基因在不同物种中的功能差异已有报道。本文分析了苹果科（Malus domestica）和日本梨（Pyrus pyrifolia）两种果树的S位点。对苹果S-9单倍型317 kb区域的测序显示了两个相似的F-box基因。从苹果和日本梨的不同单倍型中分离到同源序列，发现它们都是源自Slocus的多态基因。由于每个S单倍型包含两个或三个相关基因，因此将S位点F-box兄弟基因命名为SFBB。SFBB基因在花粉中特异表达，并且SFBB基因的可变区处于正选择状态。在日本梨花柱特异性突变体S单倍型中，SFBB基因被保留。除了其多样性外，SFBB基因满足花粉S的预期特征，并在基于S- rase的GSI系统中讨论了SFBB基因作为花粉S候选基因的独特多样性。
Although recent findings suggest that the F-box genes SFB/SLF control pollen-part S specificity in the S-RNase-based gametophytic self-incompatibility (GSI) system, how these genes operate in the system is unknown, and functional variation of pollen S genes in different species has been reported. Here, we analyzed the S locus of two species of Maloideae: apple (Malus domestica) and Japanese pear (Pyrus pyrifolia). The sequencing of a 317-kb region of the apple S-9 haplotype revealed two similar F-box genes. Homologous sequences were isolated from different haplotypies of apple and Japanese pear, and they were found to be polymorphic genes derived from the Slocus. Since each S haplotype contains two or three related genes, the genes were named SFBB for S locus F-box brothers. The SFBB genes are specifically expressed in pollen, and variable regions of the SFBB genes are under positive selection. In a style-specific mutant S haplotype of Japanese pear, the SFBB genes are retained. Apart from their multiplicity, SFBB genes meet the expected characteristics of pollen S. The unique multiplicity of SFBB genes as the pollen S candidate is discussed in the context of mechanistic variation in the S-RNase-based GSI system.