Nitrogen catabolite repression of DAL80 expression depends on the relative levels of Gat1p and Ure2p production in Saccharomyces cerevisiae
Nitrogen catabolite repression of DAL80 expression depends on the relative levels of Gat1p and Ure2p production in Saccharomyces cerevisiae
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DOI：
10.1074/jbc.275.19.14408
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发表时间：
2000-05-12
影响因子：
4.8
通讯作者：
Cooper, TG
中科院分区：
文献类型：
--
作者：
Cunningham, TS;Andhare, R;Cooper, TG
GATA家族激活子(Gln3p和Gat1p)和抑制子(Dal80p和Deh1p)调控酿酒酵母对氮分解代谢抑制(NCR)敏感的转录，可能是通过与NCR敏感基因上游的GATA序列竞争结合来实现的。Ure2p不是GATA家族成员，它抑制Gln3p/Gat1p在良好氮源存在的情况下发挥作用。我们发现，GAT1和URE2的相对表达水平可以影响NCR敏感的DAL80转录。当Gat1p过度生产时，通常用氨或谷氨酰胺观察到的NCR严重减弱，这种抑制可以通过同时增加URE2的表达来克服。此外，在去抑制生长条件下，Ure2p的过量生产几乎消除了NCR敏感的转录，即以Pro作为唯一的氮源。增强的绿色荧光蛋白-Gat1p在依赖于Gat1p的转录水平高时是核的，当它被Ure2p的过度生产抑制时是胞质的。
GATA family activators (Gln3p and Gat1p) and repressors (Dal80p and Deh1p) regulate nitrogen catabolite repression (NCR)-sensitive transcription in Saccharomyces cerevisiae presumably via their competitive binding to the GATA sequences upstream of NCR-sensitive genes. Ure2p, which is not a GATA family member, inhibits Gln3p/Gat1p from functioning in the presence of good nitrogen sources. We show that NCR-sensitive DAL80 transcription can be influenced by the relative levels of GAT1 and URE2 expression. NCR, normally observed with ammonia or glutamine, is severely diminished when Gat1p is overproduced, and this inhibition is overcome by simultaneously increasing URE2 expression. Further, overproduction of Ure2p nearly eliminates NCR-sensitive transcription under derepressive growth conditions, i.e. with proline as the sole nitrogen source. Enhanced green fluorescent protein-Gat1p is nuclear when Gat1p-dependent transcription is high and cytoplasmic when it is inhibited by overproduction of Ure2p.