Lymph nodes: microstructural anatomy at MR imaging.
Lymph nodes: microstructural anatomy at MR imaging.
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淋巴结：磁共振成像的微观结构解剖。
DOI：
10.1148/radiology.178.2.1987619
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发表时间：
1991
期刊：
影响因子：
19.7
通讯作者：
J. Wittenberg
中科院分区：
文献类型：
--
作者：
A. Lee;R. Weissleder;T. Brady;J. Wittenberg
啮齿动物淋巴结的高分辨率显微磁共振 (MR) 图像与组织学研究获得的切片直接相关，以确定 MR 成像中看到的淋巴结的微观结构解剖结构，并评估新型静脉内亲淋巴细胞 MR 造影剂（超小超顺磁性氧化铁 [USPIO]）引起的信号强度变化。未增强淋巴结的高分辨率 T2 加权图像显示髓窦为低信号强度区域，滤泡为高强度结构。单次静脉注射 USPIO（160 μmol/kg）后，T1 加权和 T2 加权图像均显示髓窦中局灶信号强度损失的区域，与巨噬细胞摄取的分布相对应。淋巴滤泡的信号强度似乎没有变化，因为它们基本上没有巨噬细胞。这种显微 MR 成像模型应该为以下方面提供基础：(a) 了解对比增强的正常淋巴结与患病淋巴结模式之间的差异；(b) 指导细胞水平上新型药物靶向策略的开发。
High-resolution microscopic magnetic resonance (MR) images of rodent lymph nodes were directly correlated with sections obtained for histologic study to determine the microstructural anatomy of lymph nodes seen at MR imaging and to evaluate signal intensity changes induced by a novel intravenous lymphotropic MR contrast agent (ultrasmall superparamagnetic iron oxide [USPIO]). High-resolution T2-weighted images of unenhanced lymph nodes demonstrated medullary sinus as regions of low signal intensity and follicles as high-intensity structures. After a single intravenous administration of USPIO (160 mumol/kg), both T1-weighted and T2-weighted images showed areas of focal signal intensity loss in medullary sinuses corresponding to the distribution of uptake by macrophages. Lymph follicles appeared unchanged in signal intensity, as they are largely devoid of macrophages. This model of microscopic MR imaging should provide the basis for (a) understanding differences between patterns of contrast-enhanced normal lymph nodes and those of diseased ones and (b) guiding the development of targeting strategies for novel pharmaceuticals at the cellular level.