High-Resolution Mapping of Interstitial Telomeric Repeats in Syrian Hamster Metaphase Chromosomes
High-Resolution Mapping of Interstitial Telomeric Repeats in Syrian Hamster Metaphase Chromosomes
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DOI：
10.1159/000321676
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发表时间：
2011-01-01
影响因子：
1.7
通讯作者：
Solovjeva, L.
中科院分区：
文献类型：
--
作者：
Demin, S.;Pleskach, N.;Solovjeva, L.
对叙利亚仓鼠（Mesocricetus auratus）的中期染色体进行dapi显带，对其进行核型分析。采用端粒fitc共轭肽核酸探针荧光原位杂交技术对（TTAGGG）(n)重复序列的间质区进行定位。强荧光原位杂交信号对应于染色体2、4、14、20和x的胞内染色质带的间隙端粒重复序列。高分辨率的dapi带可以指定这些染色体的胞内染色质带的排列。版权所有：S. Karger AG，巴塞尔
Karyotype analysis of the Syrian hamster (Mesocricetus auratus) was performed after DAPI-banding of metaphase chromosomes obtained from cultivated skin fibroblasts of a newborn animal. Fluorescence in situ hybridization with telomeric FITC-conjugated peptide nucleic acid probe was applied to map interstitial blocks of (TTAGGG)(n) repeats. Strong fluorescence in situ hybridization signals corresponded to interstitial telomeric repeats in pericentromeric chromatin bands of chromosomes 2, 4, 14, 20, and X. High-resolution DAPI-banding allowed specifying the arrangement of bands in the pericentromeric regions of these chromosomes. Copyright (C) 2010 S. Karger AG, Basel