In vitro investigation of ultrasound-induced oxidative stress on human lens epithelial cells
In vitro investigation of ultrasound-induced oxidative stress on human lens epithelial cells
复制标题
DOI：
10.1016/j.bbrc.2016.11.139
复制
发表时间：
2017-01-22
影响因子：
3.1
通讯作者：
Liu, Hao-Li
中科院分区：
文献类型：
--
作者：
Rwei, Patrick;Gong, Cihun-Siyong Alex;Liu, Hao-Li
在体外研究了超声暴露对人晶状体上皮细胞 (HLE-B3) 的影响，特别是使用各种临床相关设置对超声应用时氧化应激产生的影响。除了超声波引起的热效应之外，最近还提出氧化应激是超声波对人体细胞产生影响的主要机制之一。在这项工作中，通过 MIT 测定、Live/Dead 测定、活性氧 (ROS) 和细胞内钙水平来定量和定性评估 HLE-B3 超声暴露的生物相容性水平和氧化应激的产生。氧化应激诱导传统上是通过以下方式实现的。给予H 2 O 2 ，因此给予H 2 O 2 用作本文比较的阳性对照组。鉴于 H2O2 的施用被认为是侵入性的，并且可能具有潜在的副作用，超声波作为物理刺激可能是诱导类似氧化应激环境的更安全且非侵入性的方法。超声波对细胞活力和氧化应激诱导的影响随着超声波强度的增加而增加。结果表明，与脉冲超声波相比，连续超声波对氧化应激水平有积极影响，但对细胞活力有负面影响。此外，我们的工作表明，暴露于无微泡的 58 kPa 连续超声波可以维持可接受的细胞活力，并产生与传统 H2O2 类似的氧化应激效应。总之，与传统的 H2O2 给药相比，超声波照射可以产生氧化应激。产生氧化应激的效果可通过超声参数来调节，包括脉冲波或连续波、超声强度和微泡的添加。 (C) 2016 Elsevier Inc. 保留所有权利。
The effect of ultrasound exposure on human lens epithelial cells (HLE-B3) was investigated in vitro, specifically on the generation of oxidative stress upon ultrasound application using various clinically relevant settings. In addition to ultrasound-induced heat effects, oxidative stress has been recently proposed as one of the main mechanisms for ultrasound-induced effects on human cells. In this work, the levels of biocompatibility and generation of oxidative stress by exposure of ultrasound to HLE-B3 were evaluated quantitatively and qualitatively by the MIT assay, Live/Dead assay, reactive oxygen species (ROS) and intracellular calcium level. Oxidative stress induction is traditionally achieved through. administrations of H2O2 and thus the administration of H2O2 was used as the positive control group for comparison herein. Concerning the administrations of H2O2 are considered invasive and may potentially have side effects, ultrasound as physical stimulation could be a safer and non-invasive method to induce similar oxidative stress environments. The effect of ultrasound on cell viability and induction of oxidative stress increases with ultrasound intensity. The result reveals that the continuous ultrasound has a positive impact on the oxidative stress levels but does negatively on the cell viability, as compared to the pulsed ultrasound. Furthermore, our work demonstrates that the exposure of 58 kPa continuous ultrasound without microbubbles can maintain acceptable cell viability and produce oxidative stress effects similar to the traditional administrations of H2O2. In summary, exposure of ultrasound can generate oxidative stress comparable to traditional administrations of H2O2. The effect of generating oxidative stress is adjustable through ultrasound parameters, including the pulsed or continuous wave, the intensity of ultrasound and addition of microbubbles. (C) 2016 Elsevier Inc. All rights reserved.