Analysis of Common Issues and Research Progress in Loop Mediated Isothermal Amplification
Analysis of Common Issues and Research Progress in Loop Mediated Isothermal Amplification
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发表时间：
2016-09
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通讯作者：
Hu Zongyue;Xu Zhouheng;Lu Yiyu
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作者：
Hu Zongyue;Xu Zhouheng;Lu Yiyu
环介导等温扩增（LAMP）技术是近年来发展起来的一种新的核酸体外等温扩增技术。LAMP方法具有快速、特异、灵敏、简便等优点，已广泛应用于核酸的快速检测，但仍在不断完善和发展。在本文中，我们总结了解决扩增产物污染的方法，引物设计，以避免假阳性，以及基于LAMP技术的相关技术的发展。本文可为该方法在基层的应用提供参考。
Loop mediated isothermal amplification(LAMP)technology is a newly developed isothermal amplification technology for in vitro detection of nucleic acids. Although the LAMP assay is rapid, specific, sensitive, simple and has been widely applied for rapid detection of nucleic acids, it continues to improve and develop. In this paper, we summarize approaches to addressing amplification product contamination, primer design to avoid false positives, and the development of related techniques based on LAMP technology. This paper could serve as a reference for the application of the assay at the grassroots level.