Molecular characterization by RNA‐RNA hybridization of a serotype 8 human rotavirus with “super‐short” RNA electropherotype
Molecular characterization by RNA‐RNA hybridization of a serotype 8 human rotavirus with “super‐short” RNA electropherotype
复制标题
通过 RNA-RNA 杂交对具有“超短”RNA 电泳型的血清型 8 人轮状病毒进行分子表征
DOI：
10.1002/jmv.1890300206
复制
发表时间：
1990
影响因子：
12.7
通讯作者：
O. Nakagomi
中科院分区：
文献类型：
--
作者：
A. Ohshima;Toshiaki Takagi;T. Matsuno;S. Matsuno;O. Nakagomi
一株具有超短RNA电泳型的人轮状病毒分离株(69M株)被归类为8型，这是一种新的人类血清型[Matsuno等人，1985]用32P标记的转录探针进行RNA-RNA杂交，以评估69M株与一组人和动物轮状病毒的遗传关系。69M株与亚组I、血清2型人轮状病毒有中等水平的同源性，具有较短的RNA电泳型。然而，69M株与本研究中测试的任何其他人类轮状病毒株没有明显的亲缘关系，包括最近发现的9型轮状病毒(以WI61株为代表)和具有长RNA电泳型的亚组I人类轮状病毒(以AU-1株为代表)，这些株在遗传上与其他人类轮状病毒株不同。出乎意料的是，69M探针与牛轮状病毒株(以NCDV株为代表)的双链RNA之间也发现了中等水平的同源性。
A human rotavirus isolate (the 69M strain) with a “super‐short” RNA electropherotype has been classified as serotype 8, a new human serotype [Matsuno et al., 1985] RNA‐RNA hybridzation using 32P‐labeled transcription probes was used to assess the genetic relatedness of the 69M strain to a panel of human and animal rotaviruses. The 69M strain showed a medium level of homology with subgroup I, serotype 2 human rotaviruses with short RNA electropherotypes. However, the 69M strain was not significantly related to any other human rotavirus strains tested in this study, including recently identified serotype 9 strains (represented by the WI61 strain) and subgroup I human rotaviruses with long RNA electropherotypes (represented by the AU‐1 strain) that are shown to be genetically distinct from other human rotavirus strains. Unexpectedly, a medium level of homology was also found between the 69M probe and the double‐stranded RNAs from bovine rotavirus strains (represented by the NCDV strain).