Curcumin Protects PC12 Cells from Corticosterone-Induced Cytotoxicity: Possible Involvement of the ERK1/2 Pathway
Curcumin Protects PC12 Cells from Corticosterone-Induced Cytotoxicity: Possible Involvement of the ERK1/2 Pathway
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DOI：
10.1111/j.1742-7843.2008.00369.x
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发表时间：
2009-03-01
影响因子：
3.1
通讯作者：
Li, Xuejun
中科院分区：
文献类型：
--
作者：
Gao, Min;Zhou, Hong;Li, Xuejun
抗糖皮质激素治疗对抑郁症患者有效，提示糖皮质激素在抑郁症的发生中起关键作用。我们先前的工作证明了姜黄素对大鼠和小鼠抑郁模型的治疗效果。我们鉴定了姜黄素对皮质酮诱导的PC12细胞毒性的保护作用，并探讨了这些保护作用的机制与ERK1/2在PC12细胞中的磷酸化和表达。四甲基偶氮唑盐比色法显示姜黄素对皮质酮诱导的PC12细胞毒性有明显的保护作用。浓度为10(-8)~10(-6)M的姜黄素可使PC12细胞脱离皮质酮诱导的细胞毒性。姜黄素可使细胞存活率提高20%以上。Western印迹分析显示皮质酮可促进PC12细胞ERK1/2的磷酸化，姜黄素10(-9)M~10(-6)M显著抑制皮质酮诱导的PC12细胞ERK1/2磷酸化，且呈剂量依赖关系。以上结果提示姜黄素对PC12细胞具有保护作用，其机制可能与抑制ERK的磷酸化有关。
Antiglucocorticoid therapy in depressed patients is effective, which indicates that glucocorticoids play a key role in the occurrence of depression. Our previous work demonstrated the efficacy of curcumin in treating depression in rat and mouse models. We characterized the protective effects of curcumin against corticosterone-induced cytotoxicity in PC12 cells and explored the mechanisms of these protective effects in association with the phosphorylation and expression of ERK1/2 in PC12 cells. MTT assay showed that curcumin significantly protected PC12 cells from corticosterone-induced cytotoxicity. Curcumin at concentrations from 10(-8) to 10(-6) M rescued PC12 cells from corticosterone-induced cytotoxicity. Cell viability was increased more than 20% with curcumin treatment. Western blot analysis showed that corticosterone increased ERK1/2 phosphorylation in PC12 cells and curcumin 10(-9) M to 10(-6) M significantly inhibited corticosterone-induced ERK1/2 phosphorylation in PC12 cells in a dose-dependent manner. These results suggest that curcumin is able to protect PC12 cells which may be associated with inhibition of ERK phosphorylation.