Surface modification of intraocular lenses with hyaluronic acid and lysozyme for the prevention of endophthalmitis and posterior capsule opacification
Surface modification of intraocular lenses with hyaluronic acid and lysozyme for the prevention of endophthalmitis and posterior capsule opacification
复制标题
透明质酸和溶菌酶表面修饰人工晶状体预防眼内炎和后囊混浊
DOI：
10.1039/c4ra13499k
复制
发表时间：
2015-01-01
期刊：
影响因子：
3.9
通讯作者：
Chen, Hao
中科院分区：
文献类型：
--
作者：
Wang, Bailiang;Lin, Quankui;Chen, Hao
后发性白内障是白内障手术的并发症之一，由残留的人晶状体上皮细胞（HLECs）在后囊膜上黏附与增殖所致。感染性眼内炎是另一种常见并发症，由细菌定植和生物膜形成引起。通过与N - 羟基琥珀酰亚胺（NHS）和1 - 乙基 - 3 -（3 - 二甲基氨基丙基）碳二亚胺（EDC）反应，将透明质酸 - 溶菌酶（HA - 溶菌酶）复合涂层共价接枝到聚甲基丙烯酸甲酯（PMMA）人工晶状体表面。水接触角测量和原子力显微镜（AFM）分析表明，该涂层形成了粗糙度低的高亲水性表面。由于透明质酸（HA）的亲水性，金黄色葡萄球菌和HLECs在涂有HA或HA - 溶菌酶的PMMA上的黏附显著减少。通过扫描电子显微镜（SEM）和死活细菌活力检测试剂盒测定，HA - 溶菌酶涂层因含有溶菌酶成分，对金黄色葡萄球菌具有显著的杀菌活性。兼具抗黏附和抗菌性能的HA - 溶菌酶复合涂层有望广泛应用于PMMA人工晶状体及其他生物医学植入物的表面改性。
Posterior capsule opacification is one of the complications of cataract surgery caused by the adhesion and reproduction of residual human lens epithelial cells (HLECs) on the posterior capsule. Infectious endophthalmitis is another common complication due to bacterial colonization and biofilm formation. Hyaluronic acid–lysozyme (HA–lysozyme) composite coating was covalently grafted on the surface of PMMA intraocular lenses by reaction with N-hydroxysuccinimide (NHS) and 1-etil-3-(3-dimetilaminopropil) carbodiimida (EDC). Measurements of water contact angle and AFM revealed that the coating created a highly hydrophilic surface with low roughness. Adherences of S. aureus and HLECs on PMMA with HA or HA–lysozyme coating were significantly reduced due to the hydrophilic property of HA. Bactericidal activity of HA–lysozyme coatings, measured by SEM and LIVE/DEAD bacterial viability kit, was remarkably effective against S. aureus owing to the component of lysozyme. The achievement of the HA–lysozyme composite coating with anti-adhesive and antibacterial properties can potentially be widely used on surface modification of PMMA intraocular lenses and other biomedical implants.