Preparation and application of triangular silver nanoplates/chitosan composite in surface plasmon resonance biosensing.
Preparation and application of triangular silver nanoplates/chitosan composite in surface plasmon resonance biosensing.
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DOI：
10.1016/j.aca.2013.01.034
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发表时间：
2013-03
影响因子：
6.2
通讯作者：
Jia Zhang;Ying Sun;Hua Zhang;Bo Xu;Hanqi Zhang;D. Song
中科院分区：
文献类型：
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作者：
Jia Zhang;Ying Sun;Hua Zhang;Bo Xu;Hanqi Zhang;D. Song
本文报道了利用三角银纳米板(TSNPs)来提高表面等离子体共振(SPR)生物传感器的灵敏度。将3-巯基丙酸(MPA)修饰的TSNPs与壳聚糖、戊二醛简单混合，制得TSNPs/壳聚糖复合材料。将该复合材料沉积在金膜上，作为SPR生物传感器的固定化衬底。甲基亚胺和壳聚糖聚合物保留了TSNPs的新结构，使其不受腐蚀。此外，通过戊二醛交联的壳聚糖可以通过席夫碱反应将抗体直接固定在制备的底物上。在优化的条件下，基于TSNPs/壳聚糖复合材料的生物传感器对牛免疫球蛋白在0.075~40.00μgml−1浓度范围内有较好的响应，而基于不含TSNPs的壳聚糖生物传感器的响应范围为0.6~40μgml−1，而基于Au膜的生物传感器在2.5~40μgml−1浓度范围内响应良好。
This paper reports the utilization of triangular silver nanoplates (TSNPs) to enhance the sensitivity of surface plasmon resonance (SPR) biosensor. TSNPs modified with 3-mercaptopropinic acid (MPA) were simply mixed with chitosan and glutaraldehyde to form TSNPs/chitosan composite. The composite was deposited on Au film as immobilization substrate for SPR biosensor. The novel structures of TSNPs are preserved against etching by MPA and chitosan polymer. Moreover, chitosan cross-linked by glutaraldehyde enables antibody to be immobilized on fabricated substrate directly via Schiff alkali reaction. In the optimized conditions, the resulting biosensor based on TSNPs/chitosan composite shows a satisfactory response to bovine IgG in the concentration range of 0.075–40.00μgmL−1. While the biosensor based on chitosan without TSNPs shows a response in the concentration range of 0.6–40μgmL−1and the biosensor based on Au film shows a response in the concentration range of 2.5–40μgmL−1. The experiment results show that the sensitivity of SPR biosensor based on TSNPs/chitosan composite was significantly enhanced and the immobilization procedure of antibody was simplified.