Sequence variation in the melanocortin-1 receptor gene (Mc1r) does not explain variation in the degree of melanism in a widespread amphibian
Sequence variation in the melanocortin-1 receptor gene (Mc1r) does not explain variation in the degree of melanism in a widespread amphibian
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DOI：
10.5735/086.047.0104
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发表时间：
2010-03-10
影响因子：
0.7
通讯作者：
Merila, Juha
中科院分区：
文献类型：
--
作者：
Herczeg, Gabor;Matsuba, Chikako;Merila, Juha
黑皮质素-1受体基因（Mc 1 r）的核苷酸序列变异与几种哺乳动物、鸟类和爬行动物的黑化有关，但还没有人试图了解两栖动物黑化的遗传基础。我们从普通蛙（Rana temporaria）中分离了Mc 1 r的完整编码序列（945 bp），并将预测的氨基酸序列与鱼类、爬行动物、鸟类和哺乳动物的Mc 1 r进行了比较。我们研究了核苷酸取代和背黑化水平之间的关联，从两个人群中有显着差异的黑化28个人。根据我们的研究结果，跨膜区的Mc 1 r是保守的脊椎动物。在人群比较中，我们只发现了5个同义替换的核苷酸位点，没有一个与黑化水平相关。我们的研究结果表明，其他基因或调控区域以外的编码序列的Mc 1 r是负责表达黑素在R。暂时的
Variation in nucleotide sequence of the melanocortin-1 receptor gene (Mc1r) is associated with melanism in several mammalian, avian and reptilian species, but no attempts have been made to understand the genetic underpinnings of melanism in amphibians. We isolated the complete coding sequence (945 bps) of Mc1r from the common frog (Rana temporaria) and compared the predicted amino acid sequence with that of fish, reptiles, birds and mammals. We investigated associations between nucleotide substitutions and the level of dorsal melanism among 28 individuals from two populations with pronounced differences in melanism. According to our results, the transmembrane regions of Mc1r are conserved across vertebrates. In the population comparison, we only found five nucleotide sites with synonymous substitutions; none is being associated with the level of melanism. Our results suggest that either other genes or regulatory regions outside the coding sequence of Mc1r are responsible for expression of melanism in R. temporaria.