Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110
Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110
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DOI：
10.1128/jb.185.8.2555-2562.2003
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发表时间：
2003-04-01
影响因子：
3.2
通讯作者：
Yesilkaya, H
中科院分区：
文献类型：
--
作者：
Dale, JW;Al-Ghusein, H;Yesilkaya, H
当插入序列的拷贝数少于5个时，使用IS6110对结核分枝杆菌进行分子分型显示出低区分度。利用从伦敦结核病流行病学研究中收集的此类分离株，我们显示了IS 6110模式与spoligotype和PGRS类型之间的相当程度的一致性。这表明IS6110类型主要代表不同的菌株家族，而不是通过将IS6110会聚插入到有利位置而产生的。这得到了这些菌株中IS 6110插入基因组位点鉴定的支持。综合数据能够确定这些菌株的推定进化关系，包括分别与南亚（印度和巴基斯坦），非洲和欧洲出生的患者广泛相关的三个谱系。这些谱系似乎与M截然不同。具有多个IS6110拷贝的结核分离株。
Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.