Low expression of the antibacterial factor L-amino acid oxidase in bovine mammary gland
Low expression of the antibacterial factor L-amino acid oxidase in bovine mammary gland
复制标题
牛乳腺抗菌因子L-氨基酸氧化酶的低表达
DOI：
10.1111/asj.12237
复制
发表时间：
2014
影响因子：
2
通讯作者：
Watanabe G
中科院分区：
文献类型：
--
作者：
Nagaoka K;Zhang H;Arakuni M;Taya K;Watanabe G
在小鼠中，L -氨基酸氧化酶（LAO）利用游离氨基酸产生过氧化氢，是一种经证实的乳腺抗菌因子。乳腺炎是一种细菌感染乳腺的疾病，是奶牛最常见的疾病。在这里，我们研究了LAO是否在奶牛乳腺中表达并具有抗菌作用。在奶牛乳腺中，LAO mRNA的表达水平明显低于小鼠，并且在产生过氧化氢的牛乳中未观察到LAO活性。与荷斯坦牛相同，日本黑牛的LAO mRNA表达量也较低。乳腺炎腺中LAO mRNA的表达高于泌乳腺。此外，奶牛脾脏和淋巴结中也有高水平的LAO mRNA表达。我们得出结论，与小鼠相比，奶牛乳腺中表达LAO基因的能力较低，这可能导致乳腺炎的高发。
In the mouse, L‐amino acid oxidase (LAO) produces hydrogen peroxide by utilizing free amino acids and is a proven antibacterial factor in mammary glands. Mastitis, a bacterial infection of the mammary gland, is the most frequent disease in dairy cattle. Here, we investigate whether LAO is expressed in the mammary gland of dairy cattle and is antibacterial. In dairy cattle, the expression level of LAO mRNA in the mammary gland was considerably lower than that in mice, and LAO activity was not observed in cattle milk that produced hydrogen peroxide. The expression of LAO mRNA was also low in Japanese Black cattle, the same as in Holstein cattle. A higher LAO mRNA expression was observed in the mastitis glands than in the lactating glands. Furthermore, spleen and lymph nodes expressed high levels of LAO mRNA in dairy cattle. We conclude that mammary glands in dairy cattle have lower ability to express the LAO gene compared to that in mice, which may result in a high incidence of mastitis.