Tomato yellow leaf curl Thailand virus-[Y72] from Yunnan is a monopartite begomovirus associated with DNAβ
Tomato yellow leaf curl Thailand virus-[Y72] from Yunnan is a monopartite begomovirus associated with DNAβ
复制标题
DOI：
10.1007/s11262-009-0327-4
复制
发表时间：
2009-04-01
期刊：
影响因子：
1.6
通讯作者：
Zhou, Xueping
中科院分区：
文献类型：
--
作者：
Guo, Wei;Yang, Xiuling;Zhou, Xueping
先前的研究表明，源自泰国的泰国番茄黄卷叶病毒（TYLCTHV）分离株为双部begomov，而在中国发现的7株TYLCTHV分离株均与DNA β分子相关。本研究构建了TYLCTHV分离物Y72 （TYLCTHV-[Y72]）的感染性克隆及其DNA β，验证了TYLCTHV的二分性或单分性。农接种表明，单独接种TYLCTHV-[Y72]能在本烟、粘烟和番茄植株中诱发显著症状，但与其相关的卫星DNA β共接种时，症状更为严重，与烟草卷曲茎病毒相似。Southern blot结果显示，TYLCTHV DNA β可以增加病毒在全身感染组织中的积累。因此，TYLCTHV-[Y72]是一种单一化的贝古病毒，它可能代表了一种介于需要DNA β的贝古病毒和不需要DNA β的贝古病毒之间的进化中间体。
Previous studies have shown that isolates of tomato yellow leaf curl Thailand virus (TYLCTHV) originated from Thailand are bipartite begomoviruses, while all the seven TYLCTHV isolates found in China are associated with DNA beta molecules. In this study, infectious clones of TYLCTHV isolate Y72 (TYLCTHV-[Y72]) and its DNA beta were constructed to verify the bipartite or monopartite nature of TYLCTHV. Agroinoculation showed that TYLCTHV-[Y72] alone was able to induce significant symptoms in Nicotiana benthamiana, Nicotiana glutinosa, and Solanum lycopersicum plants, but co-inoculation with its associated satellite DNA beta produced more severe symptoms, which is similar to tobacco curly shoot virus. Southern blot results showed that TYLCTHV DNA beta could increase the virus accumulation in systemically infected tissues. Thus, TYLCTHV-[Y72] is a monopartite begomovirus, which may represent an evolutionary intermediate between the begomoviruses requiring DNA beta and begomoviruses dispensable of DNA beta.