Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick
Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick
复制标题
DOI：
10.1007/s12686-009-9111-y
复制
发表时间：
2009-12-01
影响因子：
1.1
通讯作者：
de Souza, A. P.
中科院分区：
文献类型：
--
作者：
Jungmann, L.;Vigna, B. B. Z.;de Souza, A. P.
我们描述了湿臂藻（Rendle） Schweick的第一个核简单序列重复（SSRs）位点。是一种暖季草，具有兼性无融合，倍性变异（6X-9X），是热带地区重要的牧草物种。利用富集文库方法获得38对引物，其中27对引物在巴西Embrapa肉牛种质库58份中显示多态性。11个基因座在与湿纹双歧杆菌有密切亲缘关系但分类界限不明确的双纹双歧杆菌中扩增。对其他3种腕鱼的可移植性也进行了评价。所研制的微卫星对湿藻的遗传研究、系统发育评价、保护和育种应用具有潜在的应用价值。
We describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X-9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.