Calcium influx is required for endocytotic membrane retrieval.
Calcium influx is required for endocytotic membrane retrieval.
复制标题
内吞膜修复需要钙流入。
DOI：
10.1073/pnas.96.9.5019
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发表时间：
1999
影响因子：
11.1
通讯作者：
Lambert,NA
中科院分区：
文献类型：
--
作者：
Vogel,SS;Smith,RM;Baibakov,B;Ikebuchi,Y;Lambert,NA
Cells use endocytotic membrane retrieval to compensate for excess surface membrane after exocytosis. Retrieval is thought to be calcium-dependent, but the source of this calcium is not known. We found that, in sea urchin eggs, endocytotic membrane retrieval required extracellular calcium. Inhibitors of P-type calcium channels—cadmium, ω-conotoxin MVIIC, ω-agatoxin IVA, and ω-agatoxin TK—blocked membrane retrieval; selective inhibitors of N-type and L-type channels did not. Treatment with calcium ionophores overcame agatoxin inhibition in a calcium-dependent manner. Cadmium blocked membrane retrieval when applied during the first 5 minutes after fertilization, the period when the membrane potential is depolarized. We conclude that calcium influx through ω-agatoxin-sensitive channels plays a key role in signaling for endocytotic membrane retrieval.
