Measurement of wettability of the corneal epithelium
Measurement of wettability of the corneal epithelium
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角膜上皮润湿性的测量
DOI：
10.1111/j.1755-3768.1990.tb01901.x
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发表时间：
1990
影响因子：
3.4
通讯作者：
J. Tiffany
中科院分区：
文献类型：
--
作者：
J. Tiffany
采用生理盐水冲洗、黏液冲洗或擦除黏液的方法对成年兔的角膜进行冲洗。让眼角膜在空气中风干；在不同的干燥时间放置生理盐水液滴，测量其平衡接触角θ。干燥的几个阶段可以区分：1)初始阶段有多余的水存在，要么是液滴完全扩散，要么是θ值很低；2)持续20 ~ 25 min的“高原”区，θ值较低，随时间变化缓慢，反映地表固有的润湿性；3)干燥较为严重，θ急剧上升的区域。用已知θ值和生理盐水滴的表面张力的计算表，给出角膜表面张力和角膜与生理盐水之间的界面张力。乙酰半胱氨酸洗涤的角膜显示平台值约为16°，角膜表面张力为69.3 mN/m。冲洗后的角膜保持完全湿润（θ = 0°）时间超过90分钟；因此，平台值也必须接近于零，角膜表面张力约为72 mN/m。机械擦拭的角膜干得更快，θ持续上升，没有明显的平台值，表明由于细胞损伤（约40 mN/m），润湿性要低得多。
Abstract Adult rabbit corneas were saline‐rinsed, mucolytic‐rinsed, or wiped to remove mucus. The corneas were allowed to dry in air; sessile droplets of saline were placed on the surface at various drying times, and their equilibrium contact angle θ measured. Several stages of drying can be distinguished: 1) an initial period when excess water is present, with either complete spreading of droplets or very low value of θ; 2) a ‘plateau’ region lasting about 20–25 min in which θ is low and changes slowly with time, and reflects the intrinsic wettability of the surface; 3) a region when more severe drying takes place and θ rises sharply. Computed tables are used with known values of θ and the surface tension of the saline drops, to give the surface tension of the corneal surface and the interfacial tension between cornea and saline. Acetylcysteine‐washed corneas showed a plateau value of about 16° and corneal surface tension of 69.3 mN/m. Rinsed corneas remained fully wettable (θ = 0°) for longer than 90 min; hence the plateau value must also be near zero and corneal surface tension about 72 mN/m. Mechanically‐wiped corneas dried more rapidly and θ rose continuously with no marked plateau value, indicating much lower wettability due to cellular damage (about 40 mN/m).
影响因子：
4.4
作者：
Nichols,BA;Chiappino,ML;Dawson,CR
通讯作者：
Dawson,CR