Effect of butyric acid on induction of differentiation into eosinophil-like cells in human eosinophilic leukemia cells, EoL-1 cell line: possible role of granulocyte-macrophage colony-stimulating factor as an autocrine differentiating factor.
Effect of butyric acid on induction of differentiation into eosinophil-like cells in human eosinophilic leukemia cells, EoL-1 cell line: possible role of granulocyte-macrophage colony-stimulating factor as an autocrine differentiating factor.
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丁酸对人嗜酸性白血病细胞 EoL-1 细胞系诱导分化为嗜酸性粒细胞样细胞的影响：粒细胞巨噬细胞集落刺激因子作为自分泌分化因子的可能作用。
DOI：
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发表时间：
1993
影响因子：
2.8
通讯作者：
T. Shida
中科院分区：
文献类型：
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作者：
H. Saito;T. Hayakawa;H. Mita;K. Akiyama;T. Shida
EoL-1细胞在丁酸（BA）作用下可分化为成熟的嗜酸性粒细胞样细胞。分化后的细胞具有大量的颗粒，并能产生白三烯C4和嗜酸性粒细胞阳离子蛋白（ECP），向PAF、IL-5、IL-3和GM-CSF等嗜酸性粒细胞趋化因子迁移。抗GM-CSF和IL-5的单克隆抗体，尤其是抗GM-CSF的单克隆抗体，可显著降低BA诱导分化的能力，而抗IL-3的单克隆抗体则无此作用。重组人（rh）IL-3、rhGM-CSF和重组小鼠（rm）IL-5本身不具有诱导分化的能力。另一方面，这些细胞因子的存在显著（p < 0.01）增强BA诱导的细胞分化。其他细胞因子包括rhIL-1 α/β、rhIL-2、rhIL-4、rhIL-6、rhIL-8、rhTNF-α和rhIFN-γ对分化没有影响。从BA刺激的EoL-1细胞获得的细胞RNA中检测到GM-CSF和GM-CSF受体mRNA的表达。此外，在BA刺激的EoL-1细胞的培养上清中检测到约5-10 pg/ml的GM-CSF。这些结果表明，BA诱导的EoL-1细胞分化的影响下引起的GM-CSF内源性产生的这些细胞。GM-CSF似乎也是EoL-1细胞中的自分泌分化因子。
EoL-1 cells differentiated into mature eosinophil-like cells when incubated with butyric acid (BA). The differentiated cells possessed many granules that stained with Luxol fast blue and acquired the ability to produce leukotriene C4 and eosinophil cationic protein (ECP), and migrated toward some eosinophillotactic attractants, including PAF, IL-5, IL-3 and GM-CSF. Monoclonal antibody to GM-CSF and IL-5, especially GM-CSF, strongly decreased the ability of BA to induce differentiation, whereas antibody to IL-3 did not. Recombinant human (rh) IL-3, rhGM-CSF and recombinant mouse (rm) IL-5, by themselves, did not have the ability to induce differentiation. On the other hand, the presence of these cytokines significantly (p < 0.01) augmented BA-induced cell differentiation. Other cytokines including rhIL-1 alpha/beta, rhIL-2, rhIL-4, rhIL-6, rhIL-8, rhTNF-alpha and rhIFN-gamma had no effect on differentiation. Expression of GM-CSF and GM-CSF receptor mRNA was detected in cellular RNA obtained from BA-stimulated EoL-1 cells. Moreover, about 5-10 pg/ml of GM-CSF was detected in the culture supernatant of BA-stimulated EoL-1 cells. These findings suggested that BA-induced EoL-1 cell differentiation was elicited under the influence of GM-CSF endogenously produced in these cells. GM-CSF also seemed to be an autocrine differentiating factor in EoL-1 cells.