Decreased MicroRNA-221 is Associated with High Levels of TNF-α in Human Adipose Tissue-Derived Mesenchymal Stem Cells From Obese Woman
Decreased MicroRNA-221 is Associated with High Levels of TNF-α in Human Adipose Tissue-Derived Mesenchymal Stem Cells From Obese Woman
复制标题
DOI：
10.1159/000350131
复制
发表时间：
2013-01-01
影响因子：
--
通讯作者：
Juo, Suh-Hang Hank
中科院分区：
文献类型：
--
作者：
Chou, Wen-Wen;Wang, Yu-Ting;Juo, Suh-Hang Hank
目的：探讨miR-221在人脂肪组织间充质干细胞分化中的作用。此外，还探讨了miR-221与促炎标志物和脂肪因子的关系。方法：取4例肥胖女性(平均体重指数为31.7 kg/m(2))和4例消瘦女性(平均体重指数为21.5 kg/m(2))的8个脂肪组织。诱导hASCs分化，用实时定量逆转录聚合酶链式反应(Real-time RT-PCR)检测HASC分化脂肪细胞相关基因的表达。结果：在脂肪形成过程中，肥胖者HASC分化的脂肪细胞miR-221表达显著下调，且miR-221表达水平低于瘦肉者相应脂肪细胞。较高的肿瘤坏死因子-αmRNA水平与较低的miR-221水平相关。此外，脂肪细胞中miR-221水平与BMI呈负相关。结论：我们的结果支持miR-221与肥胖的发生以及肥胖相关的炎症状态之间的联系。版权所有(C)2013 S.Karger AG，巴塞尔
Aim: The present study aimed to investigate the regulation and involvement of miR-221 in the differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs). The relationships between miR-221 and pro-inflammatory markers and adipokines were also explored. Methods: Eight adipose tissues were obtained from four obese (mean body mass index (BMI) = 31.7 kg/m(2)) and four lean (mean BMI= 21.5 kg/m(2)) women. hASCs were induced to differentiate, and the related gene expression were measured in the hASC-differentiated adipocytes using real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). Results: During adipogenesis, miR-221 was significantly down-regulated; furthermore, miR-221 levels were lower in hASC-differentiated adipocytes from obese subjects than in the corresponding adipocytes from lean subjects. Higher TNF-alpha mRNA levels were associated with lower levels of miR-221. In addition, the miR-221 levels in the adipocytes were inversely correlated with BMI. Conclusion: Our results support the link between miR-221 and obesity development as well as obesity related inflammatory status. Copyright (C) 2013 S. Karger AG, Basel