Biochemical and biophysical applications of electron spin resonance.
Biochemical and biophysical applications of electron spin resonance.
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电子自旋共振的生物化学和生物物理应用。
DOI：
10.1002/9780470110492.ch5
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Swartz,SM
中科院分区：
文献类型：
--
作者：
Swartz,HM;Swartz,SM
电子自旋共振（ESR）是研究顺磁性分子（即具有未成对电子的分子）的首选方法。生物学上重要的顺磁物质包括自由基和许多过渡元素。此外，合成的稳定自由基（自旋标记物）的使用也在迅速增加，以获得各种复杂的生物化学环境（如大分子和膜）的信息，因此，ESR技术已越来越多地用于生物化学和生物物理研究，而且随着越来越多的生物科学家意识到这些技术的能力，它们的使用很可能会进一步增加。不幸的是，大多数涉及这一主题的文本和评论对这些科学家来说并不是最佳的，往往倾向于恐吓他们试图提供信息的地方。本章的目的是提供一个严格的，但易懂的介绍，这些技术和它们在生物化学和生物物理学研究中的应用。请注意术语ESR技术的使用。电子自旋源光谱学已经发展成为一个多方面的领域，它采用了几种不同的技术，这些技术具有顺磁性物质共振吸收微波的共同基础。这些技术包括双共振技术，例如ENDOR和ELDOR，快速动力学技术，饱和转移光谱和自旋捕获。本章的主要目的如下：1.提供ESR方法的能力和局限性的理解，以便读者可以决定他们是否可能与他/她的工作有关。
Electron spin resonance, ESR, is the method of choice for studying paramagnetic molecules, that is, molecules with unpaired electrons. Biologically important paramagnetic species include free radicals and many transition elements. In addition there is substantial and rapidly growing use of synthetic stable free radicals (spin labels) to obtain information on a wide variety of complex biochemical environments such as macromolecules and membranes.Therefore, ESR techniques have become increasingly used in biochemical and biophysical investigations, and most likely their use will increase further as more biologically oriented scientists become aware of the capabilities of these techniques. Unfortunately, most texts and reviews that deal with the subject are not optimal for such scientists, often tending to intimidate where they seek to inform. This chapter aims to provide this audience with a rigorous but comprehendible introduction to these techniques and their uses in biochemical and biophysical studies. Note the use of the term ESR techniques. Electron spin resource spectroscopy has developed into a multifaceted field that employs several different techniques having a common basis of resonant absorption of microwaves by paramagnetic substances. These include double-resonance techniques, for example, ENDOR and ELDOR, fast-kinetics tech-niques, saturation transfer spectroscopy, and spin trapping. The principal aims of this chapter are the following: 1. To provide an understanding of the capabilities and limitations of ESR methods so the reader can decide if they may be pertinent for his/her work.