The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria
The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria
复制标题
DOI：
10.3390/molecules24091711
复制
发表时间：
2019-05-01
期刊：
影响因子：
4.6
通讯作者：
Pecinka, Petr
中科院分区：
文献类型：
--
作者：
Bartas, Martin;Cutova, Michaela;Pecinka, Petr
局部DNA结构在调节基本细胞过程中的作用是一个新兴的研究领域。在局部非B DNA结构中，G-四链体的重要性在过去十年中得到了证明，它们的存在和功能相关性已经在许多基因组中得到了证明，包括人类。在这项研究中，我们分析了在NCBI数据库中可用的所有完整细菌基因组中G4 Hunter的G-四链体形成序列的存在和位置。G-四链体形成序列在所有物种中均被鉴定，但在不同进化群体中的频率差异显着。G-四链体形成序列在异常球菌-栖热菌亚群中的频率最高，在栖热菌亚群中的频率最低。G-四链体形成序列是非随机分布的，并且在各种进化群体中受到青睐。G-四链体形成序列富含ncRNA片段，随后是mRNA。周围序列的分析表明G-四链体形成序列周围的tRNA和调控序列。这些数据表明细菌基因组中G-四链体形成序列的独特和非随机定位。
The role of local DNA structures in the regulation of basic cellular processes is an emerging field of research. Amongst local non-B DNA structures, the significance of G-quadruplexes was demonstrated in the last decade, and their presence and functional relevance has been demonstrated in many genomes, including humans. In this study, we analyzed the presence and locations of G-quadruplex-forming sequences by G4Hunter in all complete bacterial genomes available in the NCBI database. G-quadruplex-forming sequences were identified in all species, however the frequency differed significantly across evolutionary groups. The highest frequency of G-quadruplex forming sequences was detected in the subgroup Deinococcus-Thermus, and the lowest frequency in Thermotogae. G-quadruplex forming sequences are non-randomly distributed and are favored in various evolutionary groups. G-quadruplex-forming sequences are enriched in ncRNA segments followed by mRNAs. Analyses of surrounding sequences showed G-quadruplex-forming sequences around tRNA and regulatory sequences. These data point to the unique and non-random localization of G-quadruplex-forming sequences in bacterial genomes.