Escherichia coli transformant expressing the glucose dehydrogenase gene from Bacillus megaterium as a cofactor regenerator in a chiral alcohol production system
Escherichia coli transformant expressing the glucose dehydrogenase gene from Bacillus megaterium as a cofactor regenerator in a chiral alcohol production system
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DOI：
10.1271/bbb.62.167
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发表时间：
1998-01-01
影响因子：
1.6
通讯作者：
Shimizu, S
中科院分区：
文献类型：
--
作者：
Kataoka, M;Rohani, LPS;Shimizu, S
研究了过表达来自巨大芽孢杆菌IWG 3的葡萄糖脱氢酶（GDH）基因的大肠杆菌JM 109（pGDA 2）作为辅因子再生器的用途。在4-氯-3-氧代丁酸乙酯的不对称还原反应中，coliJM 109（pKAR），E. coliJM 109（pGDA 2）能与NADP（+）和葡萄糖一起作为NADPH再生器，类似于商业上可获得的GDH。
Escherichia coli JM109 (pGDA2) overexpressing the glucose dehydrogenase (GDH) gene from Bacillus megaterium IWG3 was examined for use as a cofactor regenerator. In the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate by E. coli JM109 (pKAR) which is an aldehyde reductase-overproducing transformant, E. coli JM109 (pGDA2) can act as an NADPH regenerator with NADP(+) and glucose, similarly to commercially available GDH.