Human term placenta as a source of hematopoietic cells.
Human term placenta as a source of hematopoietic cells.
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DOI：
10.3181/0809-bc-262
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Kuypers FA
中科院分区：
文献类型：
--
作者：
Serikov V;Hounshell C;Larkin S;Green W;Ikeda H;Walters MC;Kuypers FA
脐带血（UCB）移植更广泛的临床应用的主要障碍是其有限的细胞含量。因此，小鼠胎盘组织中造血祖细胞的发现使我们研究人类胎盘是否含有造血细胞，造血部位，并开发出处理和储存胎盘造血细胞用于移植的程序。在这里，我们表明，人胎盘含有大量的CD34表达造血细胞，有可能提供一个细胞产量几倍于一个典型的脐带血收获。来自新鲜或冷冻保存的胎盘组织的细胞在培养中产生红系和髓系集落，并且在免疫缺陷小鼠中移植后也产生淋巴样细胞。提示人胎盘有可能成为异基因造血干细胞移植的重要新来源。
The main barrier to a broader clinical application of umbilical cord blood (UCB) transplantation is its limiting cellular content. Thus, the discovery of hematopoietic progenitor cells in murine placental tissue led us investigate whether the human placenta contains hematopoietic cells, sites of hematopoiesis, and to develop a procedure of processing and storing placental hematopoietic cells for transplantation. Here we show that the human placenta contains large numbers of CD34-expressing hematopoietic cells, with the potential to provide a cellular yield several-fold greater than that of a typical UCB harvest. Cells from fresh or cryopreserved placental tissue generated erythroid and myeloid colonies in culture, and also produced lymphoid cells after transplantation in immunodeficient mice. These results suggest that human placenta could become an important new source of hematopoietic cells for allogeneic transplantation.