Maize WRKY114 gene negatively regulates salt-stress tolerance in transgenic rice
Maize WRKY114 gene negatively regulates salt-stress tolerance in transgenic rice
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玉米WRKY114基因负调控转基因水稻的耐盐胁迫能力
DOI：
10.1007/s00299-019-02481-3
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发表时间：
2019-10-28
影响因子：
6.2
通讯作者：
Cai, Ronghao
中科院分区：
文献类型：
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作者：
Bo, Chen;Chen, Haowei;Cai, Ronghao
在水稻中过表达的玉米中分离的盐响应WRKY 114基因通过调节胁迫和脱落酸相关基因的表达导致盐胁迫耐受性和脱落酸敏感性的降低。WRKYs是一类重要的转录因子家族，广泛参与植物的发育、防御调控和逆境应答。本研究从玉米中克隆了WRKY转录因子WRKY 114。ZmWRKY 114的表达量在盐胁迫下下调，在阿坝处理下上调。ZmWRKY 114是酵母中不具有转录激活能力的核蛋白。酵母单杂交实验证实ZmWRKY 114具有特异性结合W盒的能力。ZmWRKY 114在水稻中的异源过表达增强了盐胁迫敏感性，如转基因植物在盐胁迫条件下具有降低的高度、根长度和存活率所示。此外，转基因植株还保持了较低的脯氨酸含量，但较高的丙二醛含量和相对漏电水平。此外，ZmWRKY 114过表达植物在幼苗生长早期对阿坝的敏感性较低。进一步的分析表明，转基因水稻在盐胁迫条件下比野生型植株积累了更高水平的阿坝。转录组和实时定量PCR分析表明，一些调控基因，这在控制植物胁迫反应和/或阿坝信号通路中发挥重要作用，受到影响ZmWRKY 114过表达时，水稻与NaCl处理。因此，ZmWRKY 114可能作为负因子通过ABA介导的途径参与盐胁迫反应。
Overexpression in rice of the isolated salt-responsive WRKY114 gene from maize resulted in decreases in both salt-stress tolerance and abscisic acid sensitivity by regulating stress- and abscisic acid-related gene expression. WRKYs are an important family of transcription factors that widely participate in plant development, defense regulation and stress responses. In this research, WRKY114 encoding a WRKY transcription factor was cloned from maize (Zea mays L.). ZmWRKY114 expression was down-regulated by salt stress but up-regulated by abscisic acid (ABA) treatments. ZmWRKY114 is a nuclear protein with no transcriptional activation ability in yeast. A yeast one-hybrid experiment confirmed that ZmWRKY114 possesses an ability to specifically bind to W-boxes. The heterologous overexpression of ZmWRKY114 in rice enhanced the salt-stress sensitivity as indicated by the transgenic plants having reduced heights, root lengths and survival rates under salt-stress conditions. In addition, transgenic plants also retained lower proline contents, but greater malondialdehyde contents and relative electrical leakage levels. Additionally, ZmWRKY114-overexpressing plants showed less sensitivity to ABA during the early seedling growth stage. Further analyses indicated that transgenic rice accumulated higher levels of ABA than wild-type plants under salt-stress conditions. Transcriptome and quantitative real-time PCR analyses indicated that a few regulatory genes, which play vital roles in controlling plant stress responses and/or the ABA signaling pathway, were affected by ZmWRKY114 overexpression when rice was treated with NaCl. Thus, ZmWRKY114 may function as a negative factor that participates in salt-stress responses through an ABA-mediated pathway.