POLYMORPHISM OF AGE-RELATED-CHANGES IN INTERLEUKIN (IL) PRODUCTION - DIFFERENTIAL CHANGES OF T HELPER SUBPOPULATIONS, SYNTHESIZING IL2, IL3 AND IL4
POLYMORPHISM OF AGE-RELATED-CHANGES IN INTERLEUKIN (IL) PRODUCTION - DIFFERENTIAL CHANGES OF T HELPER SUBPOPULATIONS, SYNTHESIZING IL2, IL3 AND IL4
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DOI：
10.1002/eji.1830200614
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发表时间：
1990-06-01
影响因子：
5.4
通讯作者：
CINADER, B
中科院分区：
文献类型：
--
作者：
KUBO, M;CINADER, B
对近交系小鼠在佛波醇肉豆蔻酸酯乙酸盐（PMA）和钙离子载体（A23187）刺激后合成白细胞介素（IL），即白细胞介素2、白细胞介素3和白细胞介素4的能力随年龄变化的品系差异进行了研究。白细胞介素2的产生在A/J、DBA/1和DBA/2小鼠中保持恒定，而在所检测的一个品系（C57BL/6J）中随年龄增长而降低。合成的年龄相关变化的品系差异未显示出年轻时的合成能力与后期降低幅度之间的关系（“经济校正”），而这种关系在其他几个系统中是可以观察到的。不同类型多态性之间的这种差异归因于合成白细胞介素2的内在能力的年龄相关缺陷，这种缺陷可能仅发生在受试品系之一C57BL/6J中。与白细胞介素2的产生相反，在受试的三个品系的小鼠中，白细胞介素3和白细胞介素4的量随年龄增长逐渐增加。老年小鼠的T细胞中产生白细胞介素3的细胞频率比幼年小鼠高。此外，在老年动物的细胞中，白细胞介素3在相对较低的PMA浓度下就可被诱导合成，这可能是T辅助细胞两个亚群的信号需求不同的结果，也是这些细胞内在特性改变的结果。由于白细胞介素3和白细胞介素4的产生随年龄增长而增加，而白细胞介素2的产生不增加，因此可以合理地得出结论，这反映了一个分泌白细胞介素3和白细胞介素4但不分泌白细胞介素2的T辅助细胞群（可能是TH2细胞）的扩增。
Inbred mice were examined for strain differences in age-associated changes in the capacity to synthesize interleukin (IL), i.e., IL 2, IL 3 and IL 4 after stimulation with phorbol myristate acetate (PMA) and calcium ionophore (A23187). Production of IL 2 remains constant in A/J, DBA/1 and DBA/2 mice and decreases with age in one of the strains examined (C57BL/6J). Strain differences in age-associated change of synthesis did not show the relation between youthful synthetic capacity and magnitude of later decrease ("economic correction") which is observed in several other systems. This difference between different types of polymorphisms is attributed to an age-associated defect in intrinsic capacity to synthesize IL 2 which may occur in only one of the tested strains, C57BL/6J. In contrast to IL 2 production, the quantities of IL 3 and IL 4 increase progressively, with advancing age, in mice of the three strains tested. T cells from old mice contain a greater frequency of cells producing IL 3, than do those of young mice. In addition, synthesis of IL 3 is induced at a relatively lower concentration of PMA in cells from old animals and this may be a consequence of different signal requirements of the two subsets of the T helper cells, but also a change in intrinsic properties of these cells. Since IL 3 and IL 4 production, but not IL 2 production, increasing with age, it is reasonable to conclude that this reflects an expansion of a T helper cell population which secretes IL 3 and IL 4, but not IL 2, presumably TH2.