An easy and economical in vitro method for the formation of Candida albicans biofilms under continuous conditions of flow.
An easy and economical in vitro method for the formation of Candida albicans biofilms under continuous conditions of flow.
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DOI：
10.4161/viru.1.6.13186
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发表时间：
2010-11
期刊：
影响因子：
5.2
通讯作者：
Priya Uppuluri;J. Lopez-Ribot
中科院分区：
文献类型：
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作者：
Priya Uppuluri;J. Lopez-Ribot
白色念珠菌可以在医疗器械上形成生物膜，这些生物膜通常由连续的体液流动滋养，并受到剪切应力的作用。虽然许多白色念珠菌生物膜研究已经使用体外静态模型进行，但在流动条件下开发的生物膜的信息更有限。我们之前已经描述了一个简单的流动生物膜模型（SFB），用于在连续介质流动条件下发展白色念珠菌生物膜。在这里，我们从方法论的角度详细叙述，这个模型可以很容易地组装使用材料通常在大多数微生物实验室。整个过程大约需要两天时间才能完成。使用该系统开发的生物膜是坚固的，特别适用于需要大量生物膜细胞进行下游分析的研究。这种方法简化了在不断补充营养物下生物膜的形成。此外，该技术模拟体内流动条件，从而使其在生理学上比目前占主导地位的静态模型更相关。
Candida albicans can develop biofilms on medical devices and these biofilms are most often nourished by a continuous flow of body fluids and subjected to shear stress forces. While many C. albicans biofilm studies have been carried out using in vitro static models, more limited information is available for biofilms developed under conditions of flow. We have previously described a simple flow biofilm model (SFB) for the development of C. albicans biofilms under conditions of continuous media flow. Here, we recount in detail from a methodological perspective, this model that can be assembled easily using materials commonly available in most microbiological laboratories. The entire procedure takes approximately two days to complete. Biofilms developed using this system are robust, and particularly suitable for studies requiring large amounts of biofilm cells for downstream analyses. This methodology simplifies biofilm formation under continuous replenishment of nutrients. Moreover, this technique mimics in vivo flow conditions, thereby making it physiologically more relevant than the currently dominant static models.