A novel vacuolar membrane H+-ATPase c subunit gene (ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae
A novel vacuolar membrane H+-ATPase c subunit gene (ThVHAc1) from Tamarix hispida confers tolerance to several abiotic stresses in Saccharomyces cerevisiae
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DOI：
10.1007/s11033-010-0189-9
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Yang, Chuanping
中科院分区：
文献类型：
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作者：
Gao, Caiqiu;Wang, Yucheng;Yang, Chuanping
植物液泡H+-ATP酶（V-ATP酶）在应对不同不利环境条件方面发挥着重要作用。在本研究中，我们从柽柳中克隆并鉴定了 V-ATPase c 亚基基因 (ThVHAc1)。推导的 ThVHAc1 氨基酸序列缺乏信号肽，并且 ThVHAc1 是具有四个跨膜区域的高度疏水性蛋白质。瞬时表达测定表明ThVHAc1-GFP融合蛋白在洋葱表皮内膜细胞上表达。实时RT-PCR表明ThVHAc1基因的表达受NaCl、NaHCO3、PEG和CdCl2胁迫诱导，在T. hispida根、茎和叶中表达。在没有应激的情况下，外源应用脱落酸（ABA）也会刺激 ThVHAc1 转录水平，表明 ThVHAc1 参与 ABA 依赖性应激信号通路。此外，表达ThVHAc1的转基因酵母增强了对盐、干旱、紫外线、氧化、重金属、寒冷和高温的耐受性。我们的结果表明，T. hispida 的 ThVHAc1 基因在该物种中发挥抗逆作用。
Plant vacuolar H+-ATPase (V-ATPase) plays an important role in response to different adverse environmental conditions. In the present study, we cloned and characterized a V-ATPase c subunit gene (ThVHAc1) from Tamarix hispida. The deduced ThVHAc1 amino acid sequence lacks a signal peptide and ThVHAc1 is a highly hydrophobic protein with four transmembrane regions. A transient expression assay showed that the ThVHAc1-GFP fusion protein is expressed on onion epidermal endomembrane cells. Real-time RT-PCR demonstrated that ThVHAc1 gene expression was induced by NaCl, NaHCO3, PEG and CdCl2 stress in T. hispida roots, stems and leaves. Exogenous application of abscisic acid (ABA) also stimulated ThVHAc1 transcript levels in the absence of stress, suggesting that ThVHAc1 is involved in ABA-dependent stress signaling pathway. Furthermore, the transgenic yeast expressing ThVHAc1 increased salt, drought, ultraviolet (UV), oxidative, heavy metal, cold and high temperature tolerance. Our results suggested that the ThVHAc1 gene from T. hispida serves a stress tolerance role in the species.