Change in laser-induced arterial fluorescence during ablation of atherosclerotic plague.
Change in laser-induced arterial fluorescence during ablation of atherosclerotic plague.
复制标题
动脉粥样硬化斑块消融过程中激光诱导动脉荧光的变化。
DOI：
10.1002/lsm.1900090205
复制
发表时间：
1989
影响因子：
2.4
通讯作者：
Deckelbaum,LI
中科院分区：
文献类型：
--
作者：
Cutruzzola,FW;Stetz,ML;O'Brien,KM;Gindi,GR;Laifer,LI;Garrand,TJ;Deckelbaum,LI
斑块消融过程中动脉荧光变化的分析可以为开发能够选择性斑块消融而无血管穿孔风险的荧光引导消融系统提供基础。因此，荧光光谱记录从91个正常和91个动脉粥样硬化标本的尸体人主动脉。382 nm与430 nm处的激光诱导荧光强度比（LIF比）能够以89%的准确度对这些标本进行分类，阈值为1.8（动脉粥样硬化斑块1.8，正常<1.8）。为了表征斑块消融过程中荧光的变化，对16个动脉粥样硬化主动脉标本进行了冷切片机机械斑块消融。在每100 μm斑块消融后连续记录荧光光谱;记录显示荧光光谱从动脉粥样硬化到正常模式的变化。当LIF比值为1.8时，斑块消融信号终止，15个动脉粥样硬化斑块的残余斑块厚度小于200 μm; 1个样本的残余斑块厚度为300 μm。没有样本显示介质消融。LIF比值与斑块厚度有显著相关性（r = 0.73，P <0.001），但在不同厚度下，LIF比值存在显著差异。因此，激光诱导荧光光谱能够区分动脉粥样硬化与正常主动脉，并发出斑块消融完成的信号。
Analysis of the change in arterial fluorescence during plaque ablation may provide the basis for developing a fluorescence guided ablation system capable of selective plaque ablation without risk of vessel perforation. Accordingly, fluorescence spectra were recorded from 91 normal and 91 atherosclerotic specimens of cadaveric human aorta. The ratio of the laser‐induced fluorescence intensity at 382 nm to 430 nm (LIF ratio) was capable of classifying these specimens with an 89% accuracy with a threshold value of 1.8 (atherosclerotic ⩾1.8, normal <1.8). To characterize the change in fluorescence during plaque ablation, mechanical plaque ablation with a cold microtome was performed on 16 atherosclerotic aortic specimens. Fluorescence spectra were recorded serially after each 100 μm of plaque ablation; recordings revealed a change in fluorescence spectra from atherosclerotic to a normal pattern. With an LIF ratio of 1.8 to signal termination of plaque ablation, 15 of the atherosclerotic plaques had a residual plaque thickness less than 200 μm; one specimen had a residual plaque thickness of 300 μm. No specimen demonstrated ablation of the media. There was a statistically significant correlation between LIF ratio and plaque thickness (r = .73,P< .001), but considerable variation in LIF ratio existed at each thickness. Therefore, laser‐induced fluorescence spectroscopy is capable of discriminating atherosclerotic from normal aorta and of signaling completion of plaque ablation.