Overexpression of an extraplastidic β-amylase which accumulates in the radish taproot influences the starch content of Arabidopsis thaliana
Overexpression of an extraplastidic β-amylase which accumulates in the radish taproot influences the starch content of Arabidopsis thaliana
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萝卜主根中积累的质体外β-淀粉酶的过度表达影响拟南芥的淀粉含量
DOI：
10.5511/plantbiotechnology.12.1002a
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发表时间：
2012
影响因子：
1.6
通讯作者：
M. Hara
中科院分区：
文献类型：
--
作者：
I. Takahashi;T. Kuboi;T. Fujiwara;M. Hara
最近的研究表明，位于质体中的β-淀粉酶（EC 3.2.1.2）参与内源淀粉的降解。另一方面，许多植物具有相当数量的质体外β-淀粉酶。由于它们存在于质体之外，人们认为质外β-淀粉酶不会促进植物中的淀粉降解。研究了萝卜（Raphanus sativus）主要的质外β-淀粉酶（RsBAMY 1）在淀粉代谢中的作用。淀粉酶活性在萝卜主根生长过程中升高。在淀粉酶活性升高过程中，淀粉含量下降，麦芽糖、葡萄糖、果糖和蔗糖等可溶性糖含量增加。在萝卜主根中，RsBAMY 1蛋白主要积累在贮藏薄壁组织的初生形成层和异常形成层中。而淀粉粒则位于形成层内部，与异常形成层相邻。当RsBAMY 1基因在拟南芥中过表达时，植物的淀粉含量低于野生型植物。这些结果表明，在某些植物中，质体外β-淀粉酶可能影响淀粉的代谢。
Recent studies have revealed that β-amylases (EC 3.2.1.2) located in plastids participate in endogenous starch degradation. On the other hand, many plants possess a respectable amount of extraplastidic β-amylases. Because of their existence outside of the plastids, it has been believed that extraplastidic β-amylases do not contribute to starch degradation in plants. Here, we investigated the role of the major extraplastidic β-amylase of the radish (Raphanus sativus) (RsBAMY1) in starch metabolism. Amylase activity was elevated in the growing taproot of the radish. During the elevation of amylase activity, the starch content decreased, but the contents of soluble sugars such as maltose, glucose, fructose and sucrose increased. In the radish taproot, RsBAMY1 protein accumulated in the primary cambium and anomalous cambium of the storage parenchyma. The starch granules, however, were found just inside the cambium and adjacent to the anomalous cambium. When the RsBAMY1 gene was overexpressed in Arabidopsis, the plants contained lower starch contents than the wild-type plant. These results suggest that the extraplastidic β-amylase may affect the starch metabolism in some plants.
利用拟南芥阿拉伯半乳聚糖蛋白 21 基因的 5-非翻译区在双子叶植物中高水平表达转基因
影响因子：
1.6
作者：
Matsui;T.;Matsuura;H.;Sawada;K.;Takita;E.;Kinjo;S.;Takenami;S.;Ueda;K.;Nishigaki;N.;Yamasaki;S.;Hata;K.;Yamaguchi;M.;Demura;T. and Kato;K
通讯作者：
K
影响因子：
9.2
作者：
Chiu, WL;Niwa, Y;Sheen, J
通讯作者：
Sheen, J
影响因子：
2.8
作者：
Sugio, Tadatoshi;Satoh, Junko;Kato, Ko
通讯作者：
Kato, Ko