Critical roles of neutrophils in host defense against experimental systemic infections of mice by Listeria monocytogenes, Salmonella typhimurium, and Yersinia enterocolitica
Critical roles of neutrophils in host defense against experimental systemic infections of mice by Listeria monocytogenes, Salmonella typhimurium, and Yersinia enterocolitica
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DOI：
10.1128/iai.65.2.630-635.1997
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发表时间：
1997-02-01
影响因子：
3.1
通讯作者：
Conlan, JW
中科院分区：
文献类型：
--
作者：
Conlan, JW
这项研究表明，中性粒细胞是至关重要的打击实验性全身感染小鼠的细菌病原体李斯特菌，鼠伤寒沙门氏菌，小肠结肠炎耶尔森氏菌。它表明，通过用粒细胞消耗单克隆抗体RB 6 - 8 C5治疗而导致血小板减少的小鼠比免疫活性小鼠更容易感染这三种病原体中的每一种。与免疫活性小鼠相比，血小板减少小鼠在抗菌能力方面表现出几个缺陷。首先，在免疫活性小鼠中发生的最初植入肝脏和脾脏的李斯特菌、沙门氏菌或耶尔森氏菌的立即灭活在免疫活性小鼠的这些器官中被消除。其次，与免疫活性小鼠不同，免疫活性小鼠既不能控制随后接种的细菌在肝脏和脾脏中的增殖，也不能防止感染传播到其他器官。第三，呈现血小板减少的小鼠对这三种感染产生全身性白细胞减少症。总体而言，本研究表明，中性粒细胞执行多种抗菌功能，这些功能相结合，严重限制了李斯特菌、沙门氏菌和耶尔森氏菌在感染的免疫前阶段在组织中繁殖的速率，从而为宿主提供了发展和表达更有效的特异性保护性免疫的机会。
This study shows that neutrophils are critical for combating experimental systemic infections of mice by the bacterial pathogens Listeria monocytogenes, Salmonella typhimurium, and Yersinia enterocolitica. It shows that mice rendered neutropenic by treatment with the granulocyte-depleting monoclonal antibody RB6-8C5 are far more susceptible than immunocompetent mice to infection with each of these three pathogens. Compared to immunocompetent mice, neutropenic mice exhibit several defects in their antibacterial capabilities. Firstly, the immediate inactivation of Listeria, Salmonella, or Yersinia that initially implants in the livers and spleens that occurs in immunocompetent mice is abolished in these organs in neutropenic mice, Secondly, unlike immunocompetent mice, neutropenic mice neither control the subsequent proliferation of the inoculated bacteria in the livers and spleens nor prevent dissemination of infection to other organs. Thirdly, mice rendered neutropenic develop a generalized leukopenia in response to these three infections, Overall, this study indicates that neutrophils perform diverse antimicrobial functions that, combined, severely restrict the rate at which Listeria, Salmonella, and Yersinia multiply in the tissues during the preimmune phase of infection and thereby provide the host with the opportunity to develop and express more efficient specific protective immunity.