Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase -: Evidence of a bridging inorganic sulfur
Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase -: Evidence of a bridging inorganic sulfur
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DOI：
10.1074/jbc.m008617200
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发表时间：
2000-12-29
影响因子：
4.8
通讯作者：
Cambillau, C
中科院分区：
文献类型：
--
作者：
Brown, K;Djinovic-Carugo, K;Cambillau, C
一氧化氮还原酶（N2 OR）催化N2 O的双电子还原为N-2。在2.4埃和1.6埃分辨率下分别解析了海洋假单胞菌（Pn）和海洋副球菌（Pd）的N（2）ORs的晶体结构。Pn N2 OR结构表明催化CuZ中心属于一种新型的金属簇合物，其中4个铜离子由7个组氨酸残基配位。提出了桥接氧部分和两个其它氢氧化物配体以完成连接方案（Brown，K.，Tegoni，RI. Prudencio，M.，佩雷拉，A.美国，贝松，S.，莫拉，J.J.G.，莫拉岛，和Cambillau，C.，（2000）Nat. Struct Biol.7，191-195）。然而，在CuZ簇中，无机硫的化学测定和Pd N2 OR的高分辨率结构确定了桥接无机硫而不是氧，这一结果与迄今为止令人困惑的光谱数据相一致。
Nitrous-oxide reductases (N2OR) catalyze the two-electron reduction of N2O to N-2. The crystal structure of N(2)ORs from Pseudomonas nautica (Pn) and Paracoccus denitrificans (Pd) were solved at resolutions of 2.4 and 1.6 Angstrom respectively. The Pn N2OR structure revealed that the catalytic CuZ center belongs to a new type of metal cluster in which four copper ions are liganded by seven histidine residues. A bridging oxygen moiety and two other hydroxide ligands were proposed to complete the ligation scheme (Brown, K., Tegoni, RI., Prudencio, M., Pereira, A. S., Besson, S., Moura, J. J. G., Moura, I., and Cambillau, C, (2000) Nat. Struct Biol. 7, 191-195). However, in the CuZ cluster, inorganic sulfur chemical determination and the high resolution structure of Pd N2OR identified a bridging inorganic sulfur instead of an oxygen, This result reconciles the novel CuZ cluster with the hitherto puzzling spectroscopic data.