Disconnection of the hippocampal-perirhinal cortical circuits severely disrupts object-place paired associative memory.
Disconnection of the hippocampal-perirhinal cortical circuits severely disrupts object-place paired associative memory.
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DOI：
10.1523/jneurosci.1580-10.2010
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发表时间：
2010-07-21
期刊：
影响因子：
--
通讯作者：
Lee I
中科院分区：
文献类型：
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作者：
Jo YS;Lee I
海马和嗅周皮层（perirhinal cortex，PR）之间通过内嗅皮层直接或间接地连接。虽然已经假设这两个区域应该在解剖连接的基础上彼此具有密切的功能相互作用，但许多损伤研究已经证明海马和PR之间的功能分离。为了显示这两个区域之间的紧密功能关系，我们使用了针对受试者内海马和PR的可逆失活技术，结合双连续记忆任务，其中大鼠必须考虑关于物体及其位置的信息。具体而言，大鼠植入两套双边套管到海马和PR，并在径向迷宫中的对象-位置配对关联任务进行了测试。当在两个手臂之间交替时，老鼠被要求选择一个与给定手臂唯一相关的物体作为食物。双侧蝇蕈醇（MUS）注射到海马或PR同样产生机会水平的性能。当一个功能断开程序被用来破坏海马和PR之间的相互作用，对侧MUS注射到海马和PR导致严重损害的性能。然而，失活海马和PR同侧不影响性能。在一个简单的物体辨别任务中，与MUS相同的功能性断开协议并不影响性能。结果有力地表明，海马体，PR，以及它们的功能相互作用都是不可或缺的，当对象和它们的空间位置必须同时处理。
The hippocampus and the perirhinal cortex (PR) are reciprocally connected both directly and indirectly via the entorhinal cortex. Although it has been hypothesized that the two regions should have intimate functional interactions with each other on the basis of the anatomical connectivity, many lesion studies have demonstrated functional dissociations instead between the hippocampus and PR. To show a tight functional relationship between the two regions, we used reversible inactivation techniques targeting both the hippocampus and PR within subjects, combined with a biconditional memory task in which the rat must consider information about objects and their locations. Specifically, rats were implanted with two sets of bilateral cannulas into the hippocampus and PR, and were tested in an object-place paired-associate task in a radial maze. While alternating between two arms, the rats were required to choose one of the objects exclusively associated with a given arm for food. Bilateral muscimol (MUS) injections into either the hippocampus or PR equally produced chance level performance. When a functional disconnection procedure was used to disrupt the interaction between the hippocampus and PR, contralateral MUS injections into the hippocampus and PR resulted in severe impairment in performance. However, inactivating the hippocampus and PR ipsilaterally did not affect the performance. In a simple object discrimination task, the same functional disconnection protocol with MUS did not affect the performance. The results powerfully demonstrate that the hippocampus, the PR, and their functional interactions are all indispensable when objects and their spatial locations must be processed at the same time.