Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis
Increased mucin-degrading bacteria by high protein diet leads to thinner mucus layer and aggravates experimental colitis
复制标题
高蛋白饮食增加粘蛋白降解细菌导致粘液层变薄并加重实验性结肠炎
DOI：
10.1111/jgh.15562
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发表时间：
2021-06-16
影响因子：
4.1
通讯作者：
Liu, Xiaowei
中科院分区：
文献类型：
--
作者：
Chen, Lulu;Wang, Jingyan;Liu, Xiaowei
背景与目的西方化的高脂饮食增加了炎症性肠病（IBD）的风险，但对高蛋白饮食的作用认识不足。方法雌性BALB/c小鼠分别饲喂标准饲料、高酪蛋白饲料（HCD）、高乳清蛋白饲料和高大豆蛋白饲料4周，观察不同蛋白质（酪蛋白、乳清蛋白、大豆蛋白）组成的高蛋白饲料对实验性结肠炎的治疗作用及其对结肠黏膜微生物群、结构和功能的影响。比较了不同饮食干预后小鼠对葡聚糖硫酸钠（DSS）诱导的结肠炎的易感性和结肠粘液层厚度，并鉴定了广谱抗生素干预（饮用水中含0.5 g/L万古霉素和1 g/L硫酸新霉素、甲硝唑和氨苄青霉素）的逆转作用。进一步分析了参与粘液层降解的粘蛋白、微生物群和唾液酸酶的合成，结果高蛋白饮食加重了与蛋白质组成无关的DSS诱导的急性结肠炎，而广谱抗生素逆转了这种作用。HCD显著改变了结肠粘液层细菌的组成，尤其是多形拟杆菌和总粘蛋白降解菌;此外，HCD还增加了唾液酸酶的浓度，降低了粘液层的厚度。然而，它对Muc 2的合成没有表现出显著的影响。结论高蛋白饮食改变了结肠粘膜的微生物组成和粘膜层厚度，导致DSS诱导的急性结肠炎加重。
Background and Aim Westernized high-fat diet increases the risk for inflammatory bowel diseases (IBDs), yet with insufficient understanding of the role of high-protein diet. We aimed to identify the effect of high-protein diets from different dietary proteins (casein, whey protein, soy protein) on experimental colitis and its impact on microbiota, structure and function of colonic mucus layer.Methods Female BALB/c mice were fed by standard diet, high-casein diet (HCD), high whey protein diet or high soy protein diet for 4 weeks. The susceptibility of dextran sulfate sodium (DSS)-induced colitis in mice and thickness of colonic mucus layer were compared after different dietary interventions, associated with the identification of the reversal effect of broad-spectrum antibiotic intervention (0.5 g/L of vancomycin and 1 g/L of neomycin sulfate, metronidazole and ampicillin in drinking water). Further analysis was performed on the synthesis of mucin, microbiota and sialidase involved in degradation of mucus layer.Results High-protein diets aggravated acute DSS-induced colitis independent of protein composition, while broad-spectrum antibiotics reversed this effect. HCD significantly altered the composition of bacteria in the colonic mucus layer, especially Bacteroides thetaiotaomicron and total mucin-degrading bacteria; besides, it increased sialidase concentration and reduced the thickness of mucus layer. However, it exhibited no significant effect on the synthesis of Muc2. Broad-spectrum antibiotics decreased the abundance of mucin-degrading bacteria and sialidase concentration while increased the thickness of mucus layer.Conclusion High-protein diet shifts microbial composition and thickness of colonic mucus layer, leading to the aggravation of acute DSS-induced colitis.