Phylogenetic analysis of Spiroplasmas from three freshwater crustaceans (Eriocheir sinensis, Procambarus clarkia and Penaeus vannamei) in China
Phylogenetic analysis of Spiroplasmas from three freshwater crustaceans (Eriocheir sinensis, Procambarus clarkia and Penaeus vannamei) in China
复制标题
中国三种淡水甲壳动物（中华绒螯蟹、克氏原螯虾和南美白对虾）螺原体的系统发育分析
DOI：
10.1016/j.jip.2008.06.008
复制
发表时间：
2008-09-01
影响因子：
3.4
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Bi, Keran;Huang, Hua;Wang, Wen
近年来，随着我国淡水养殖业的快速发展，淡水养殖甲壳类（蟹、虾、螯虾）的动物流行性疾病引起了人们的高度重视。螺旋体是上述三种淡水甲壳动物的致死性病原体。本研究对这些淡水甲壳动物螺原体菌株的进一步特征进行了分析。通过对16 S rRNA、gyrB和rpoB基因部分核苷酸序列的分析，结合23 S rRNA基因和16 S-23 S rRNA基因间间隔区（ISRs）的全序列测定，研究了它们的系统发育位置。系统发育分析表明，上述3个淡水甲壳动物螺原体菌株序列完全相同，与奇异螺原体亲缘关系较近。此外，基因组大小，血清学研究和实验感染特性证实了三个淡水甲壳动物螺原体菌株是一个单一的物种，而不是传统的S。奇迹。因此，这些数据表明，一个单一的物种的螺原体感染所有三个调查的淡水甲壳动物在中国，是一个潜在的候选人，一个新的物种在螺原体属。这些结果提供了重要的信息，为进一步调查在新鲜水产养殖动物的震颤疾病，由这种传染性病原体引起的。(C)2008年爱思唯尔公司All rights reserved.
Disease epizootics in freshwater culture crustaceans (crab, crayfish and shrimp) gained high attention recently in China, due to intensive developments of freshwater aquacultures. Spiroplasma was identified as a lethal pathogen of the above three freshwater Crustaceans in previous studies. Further characterization of these freshwater crustacean Spiroplasma strains were analyzed in the Current study. Phylogenetic position was investigated by analysis of partial nucleoticle sequences of 16S ribosomal RNA (rRNA), gyrB and rpoB genes, together with complete sequencing of 23S rRNA gene and 16S-23S rRNA intergenetic spacer regions (ISRs). Phylogenetic analysis of these sequences showed that the above-mentioned three freshwater crustacean Spiroplasma strains were identical and had a close relationship with Spiroplasma mirum. Furthermore, the genomic size, serological studies and experimental infection characteristics confirmed that three freshwater crustacean Spiroplasma strains are a single species other than traditional S. mirum. Therefore, these data Suggest that a single species of Spiroplasma infects all three investigated freshwater crustaceans in China, and is a potential candidate for a new species within the Spiroplasnia genus. These results provide critical information for the further investigations in fresh aquaculture epizootics related to tremor diseases, caused by this infectious agent. (C) 2008 Elsevier Inc. All rights reserved.