VINCULIN, AN INTRACELLULAR PROTEIN LOCALIZED AT SPECIALIZED SITES WHERE MICROFILAMENT BUNDLES TERMINATE AT CELL-MEMBRANES
VINCULIN, AN INTRACELLULAR PROTEIN LOCALIZED AT SPECIALIZED SITES WHERE MICROFILAMENT BUNDLES TERMINATE AT CELL-MEMBRANES
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DOI：
10.1073/pnas.77.7.4127
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
SINGER, SJ
中科院分区：
文献类型：
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作者：
GEIGER, B;TOKUYASU, KT;SINGER, SJ
该实验室最近从鸡砂囊平滑肌中分离出一种 130,000 MW 的细胞内蛋白。对培养的鸡成纤维细胞的免疫荧光观察表明，它集中在细胞的腹侧表面，在那里它们与基质形成粘斑。粘着斑是成纤维细胞内部微丝束终止于细胞膜的部位。这种新蛋白质（本文称为纽蛋白）可能参与各种细胞类型中微丝束末端与膜的连接。为了探索这种可能性，通过免疫电子显微镜标记标本超薄冷冻切片上的纽蛋白来检查几种鸡组织，包括肠上皮、砂囊平滑肌和心肌横纹肌。在每种情况下，纽蛋白的免疫标记都集中在微丝束终止的膜位点附近：在上皮细胞刷状缘连接复合物中粘附的小带处；平滑肌细胞膜相关致密斑块；筋膜处粘附有心肌细胞的闰盘膜。纽蛋白可能参与微丝束锚定到各种细胞的特定膜位点。
An intracellular protein of 130,000 MW was recently isolated in this laboratory from chicken gizzard smooth muscle. As shown by immunofluorescence observations of cultured chicken fibroblasts, it was concentrated on the ventral surfaces of the cells where they formed focal adhesions to the substratum. Focal adhesions are sites where, inside the fibroblast, microfilament bundles are known to terminate at the cell membrane. This new protein (herein named vinculin) might be involved in the linkage of the termini of microfilament bundles to membranes in various cell types. To explore this possibility, several chicken tissues, including intestinal epithelium, gizzard smooth muscle and cardiac striated muscle were examined by immunoelectron microscopic labeling for vinculin on ultrathin frozen sections of the specimens. In each case, the immunolabeling for vinculin was concentrated close to membrane sites where microfilament bundles terminate: at the zonula adherens in the junctional complex of the brush border of epithelial cells; at the membrane-associated dense plaques of smooth muscle cells; and at the fascia adherens of the intercalated disk membranes of cardiac muscle cells. Vinculin may participate in the anchoring of microfilament bundles to specific membrane sites in various cells.