Systematic investigation of Amphioxus (Branchiostoma floridae) microRNAs.
Systematic investigation of Amphioxus (Branchiostoma floridae) microRNAs.
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DOI：
10.1016/j.gene.2012.06.065
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发表时间：
2012-10
期刊：
影响因子：
3.5
通讯作者：
Xue Zhou;P. Jin;Sheng Qin;Liming Chen;Fei Ma
中科院分区：
文献类型：
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作者：
Xue Zhou;P. Jin;Sheng Qin;Liming Chen;Fei Ma
microRNAs（miRNAs）通过调控基因活性参与多种生物学过程。文昌鱼是最好的替代品作为脊椎动物的近缘无脊椎动物的祖先。本文对文昌鱼miRNAs进行了系统的研究。首先，我们鉴定了245个候选miRNAs，其中183个miRNAs为首次报道。其次，我们提供了证据支持miRNA基因在某些家族中的出生和死亡过程，并对miRNA在进化过程中的功能多样性提供了启示。第三，我们鉴定了47个发育特异性表达的miRNAs。我们发现只有19个miRNAs在所有发育阶段表达，16个miRNAs是神经元特异性的，13个miRNAs是幼虫特异性的。此外，这些潜在的miRNA靶向基因主要分为发育、肌肉形成、细胞粘附和基因调控等类别。最终在文昌鱼中发现了136个miRNAs靶向的79个免疫相关基因。总之，我们的研究结果对文昌鱼miRNAs的功能和进化都有深入的了解。
MicroRNAs (miRNAs) participate in various biological processes via controlling gene activity. Amphioxus is the best available stand-in as the proximate invertebrate ancestor of the vertebrates. Here, we systematically investigated the miRNAs in amphioxus. First, we identified 245 candidate amphioxus miRNAs, in which 183 miRNAs were firstly reported. Second, we gave evidences to support a birth-and-death process of miRNA genes in some families and gave implications for the functional diversification of miRNA during evolution. Third, we identified 47 development-specific expression miRNAs. We found that only 19 miRNAs were expressed in all developmental stages, 16 miRNAs were neurula-specific and 13 miRNAs were larva-specific. In addition, these potential miRNA-targeting genes were mainly classified into development, muscle formation, cell adhesion, and gene regulation categories. Finally, we found 79 immune related genes targeted by 136 miRNAs in amphioxus. In conclusion, our results take an insight into both the function and evolution of the amphioxus miRNAs.