NONINVASIVE FLUORESCENCE DETECTION OF HEPATIC AND RENAL FUNCTION
NONINVASIVE FLUORESCENCE DETECTION OF HEPATIC AND RENAL FUNCTION
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DOI：
10.1117/1.429854
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发表时间：
1998-07-01
影响因子：
3.5
通讯作者：
Jones, William B.
中科院分区：
文献类型：
--
作者：
Dorshow, Richard B.;Bugaj, Joseph E.;Jones, William B.
采用非侵入性体内荧光检测方案连续监测外源性染料从血管系统的清除。在大鼠模型中，肝脏和肾脏的正常和受损生理功能之间的区别得到了证明。一根光纤将光源发出的光传输到耳朵;另一根光纤位于耳朵附近，将荧光传输到检测器系统。在该初始研究中采用了两种模型染料系统。用780 nm的激光在体内激发已知仅由肝脏从血流中清除的吲哚菁绿色。在830 nm处检测荧光信号。获得了正常肝功能的特征清除曲线。在肝脏部分切除术后，清除曲线随时间延长，这与肝功能下降的预期一致。此外，荧光素标记的聚-D-赖氨酸，一种主要由肾脏从血流中清除的小聚合物，在体内用488 nm的激光激发。在518 nm处检测荧光信号。获得了正常肾功能的特征清除率曲线。在双侧肾脏结扎后，清除率曲线保持升高和恒定，表明几乎没有清除率。因此，建立了一种新的无创生理功能评估方法的可行性。(C)1998年，由光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）主办。
A noninvasive in vivo fluorescence detection scheme was employed to continuously monitor exogenous dye clearance from the vasculature. Differentiation between normal and impaired physiological function in a rat model was demonstrated for both liver and kidney. A fiber optic transmitted light from source to ear; a second fiber optic positioned near the ear transmitted the fluorescent light to a detector system. Two model dye systems were employed in this initial study. Indocyanine green, known to be exclusively cleared from the blood stream by the liver, was excited in vivo with laser light at 780 nm. The fluorescence signal was detected at 830 nm. A characteristic clearance curve of normal hepatic function was obtained. After a partial hepatectomy of the liver, the clearance curve was extended in time, as would be expected from reduced hepatic function. In addition, fluorescein labeled poly-D-lysine, a small polymer predominantly cleared from the blood stream by the kidney, was excited in vivo with laser light at 488 nm. The fluorescence signal was detected at 518 nm. A characteristic clearance curve of normal renal function was obtained. After a bilateral ligation of the kidneys, the clearance curve remained elevated and constant, indicating little if any clearance. Thus, the feasibility of a new noninvasive method for physiological function assessment was established. (C) 1998 Society of Photo-Optical Instrumentation Engineers.