Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth
Quantitative analysis of the calcium and phosphorus content of developing and permanent human teeth
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DOI：
10.1016/j.aanat.2006.09.008
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Gaengler, P.
中科院分区：
文献类型：
--
作者：
Arnold, W. H.;Gaengler, P.
本研究采用EDX元素分析方法研究了Ca、P、C在人牙芽和恒牙的牙本质、牙本质和牙釉质中的分布。一个16周大的人类胎儿下颌骨含有8颗矿化牙芽和3颗人类恒磨牙，用甲醛固定并嵌入Technovit 9100。在下颌骨正背方向连续切取厚度为80 μ m的切片，并对切片进行偏振紧密显微镜观察。恒牙在中-远端方向切牙。采用扫描电子显微镜和EDX元素分析，使用Philips XL 30 FEG扫描显微镜和EDAX能量色散x射线系统进行点测量、EDX线扫描和元素测绘。定量测定牙本质、牙本质相邻矿化牙本质、成熟牙本质、发育牙的矿化牙釉质、幼龄牙釉质和恒牙的成熟牙釉质。在牙齿发育过程中，钙、磷含量由外牙本质向矿化牙本质迅速增加。在发育中的牙釉质棱柱中，钙、磷含量从表面向牙釉质交界处呈线性增加。在恒牙中只发现了一小层预备牙本质。恒牙釉质和牙髓周围牙本质中钙、磷含量高于发育牙。牙本质前区和牙本质区Ca/P比值不同，反映不同的磷酸钙成分，但矿化区和幼牙釉质区Ca/P比值相同。钙、磷分布的差异反映了釉质和牙本质基质矿化模式的不同。(c) 2006 Elsevier GmbH版权所有。
It was the aim of this study to investigate the distribution of Ca, P and C in predentin, dentin and enamel in human tooth buds and permanent teeth by EDX element analysis. The mandible of a 16-week-old human fetus containing eight mineralizing tooth buds and three human permanent molars were fixed in formaldehyde and embedded in Technovit 9100. Serial sections of 80 mu m thickness of the mandible were cut in the frontal-dorsal direction, and polarized tight micrographs were taken of these sections. The permanent teeth were cut in mesio-distal direction. The sections were investigated with scanning electron microscopy and EDX element analysis with a Philips XL 30 FEG scanning microscope and an EDAX energy-dispersive X-ray system using spot measurements, EDX line-scans and element mapping. Quantitative measurements were made in predentin, mineralizing dentin adjacent to predentin, mature dentin, mineralizing enamel and young enamel of developing teeth and mature enamel of permanent teeth. In developing teeth the Ca and P content increased rapidly from outer predentin towards mineralizing dentin. In enamel prisms of developing teeth the Ca and P content increased linearly from the surface towards the enamel-dentin junction. In permanent teeth only a small layer of predentin was found. The Ca and P content in enamel and circumpulpal dentin of permanent teeth was higher than in developing teeth. The Ca/P ratio differed between predentin and dentin areas reflecting different calcium phosphate compositions, but it was the same in mineralizing and young enamel. The differences in the distribution of Ca and P reflect different mineralizing patterns of the enamel and dentin matrices. (c) 2006 Elsevier GmbH. All rights reserved.