Building a KATalogue of acetyllysine targeting and function.
Building a KATalogue of acetyllysine targeting and function.
复制标题
DOI：
10.1093/bfgp/elv045
复制
发表时间：
2016-03
影响因子：
4
通讯作者：
Baetz K
中科院分区：
文献类型：
--
作者：
Downey M;Baetz K
乙酰化是一种动态的翻译后修饰，通过赖氨酸乙酰转移酶（KAT）连接到蛋白质底物上，并通过赖氨酸脱乙酰酶（KDAC）去除。虽然这些酶的最佳特征是组蛋白修饰剂和基因转录的调节剂，但在许多系统中的工作强调乙酰化是一种普遍的修饰，并表明细胞中KAT和KDAC功能的广泛范围。当我们超越生成乙酰化蛋白质列表时，乙酰化领域迫切需要强大的工具来将乙酰化和脱乙酰化机制与各自的底物连接起来，并剖析各个位点的功能。酿酒酵母模型系统在经过验证的真实遗传技术以及尖端蛋白质组学和细胞成像方法的背景下提供了这样的工具包。在这里，我们回顾了这些方法的背景下，他们的贡献乙酰化研究迄今为止，并提出解决该领域挥之不去的问题的策略。
Acetylation is a dynamic post-translational modification that is attached to protein substrates by lysine acetyltransferases (KATs) and removed by lysine deacetylases (KDACs). While these enzymes are best characterized as histone modifiers and regulators of gene transcription, work in a number of systems highlights that acetylation is a pervasive modification and suggests a broad scope for KAT and KDAC functions in the cell. As we move beyond generating lists of acetylated proteins, the acetylation field is in dire need of robust tools to connect acetylation and deacetylation machineries to their respective substrates and to dissect the function of individual sites. The Saccharomyces cerevisiae model system provides such a toolkit in the context of both tried and true genetic techniques and cutting-edge proteomic and cell imaging methods. Here, we review these methods in the context of their contributions to acetylation research thus far and suggest strategies for addressing lingering questions in the field.