Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains
Phylogenetic analysis of Escherichia coli strains causing neonatal meningitis suggests horizontal gene transfer from a predominant pool of highly virulent B2 group strains
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DOI：
10.1086/514217
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发表时间：
1998-03-01
影响因子：
6.4
通讯作者：
Denamur, E
中科院分区：
文献类型：
--
作者：
Bingen, E;Picard, B;Denamur, E
研究了全球分离的69株新生儿脑膜炎大肠杆菌的系统发育关系，比较了这些分离株的rrn操作子限制性内切片段长度多态性（rrn RFLP）与ECOR参考收集的72株的rrn RFLP， K1抗原、聚合酶链反应检测的ibe10基因、pap、afa、sfa/foc、hly和aer操作子的分布。与先前与新生儿脑膜炎相关的14.9 kb含rrna的HindIII片段进行了比较。观察到脑膜炎菌株的寡克隆性。对rm RFLP数据对应的因子分析显示，脑膜炎菌株的频率梯度从系统发育的B2组（68%）到a组（6%），再经过D组和B1组（26%），毒力决定因素的分布表明它们在大肠杆菌进化过程中的水平转移。对一些新生儿状况的分析进一步表明，新生儿脑膜炎是由毒力细菌基因和宿主因素之间的平衡造成的。
Phylogenetic relationships of 69 neonatal meningitis Escherichia coli strains isolated worldwide were studied, Restriction fragment length polymorphism of rrn operons (rrn RFLP) in these isolates was compared with that of the 72 strains of the ECOR reference collection, Distributions of K1 antigen, of polymerase chain reaction-detected ibe10 gene, pap, afa, sfa/foc, hly, and aer operons, and of a 14.9-kb rrn-containing HindIII fragment previously associated with neonatal meningitis were compared. Oligoclonality was observed for the meningitis strains. Factorial analysis of correspondence on the rm RFLP data showed a frequency gradient of meningitis strains from the phylogenetic B2 group (68%) to the A group (6%), via the D and B1 groups (26%), The distribution of the virulence determinants argues for their horizontal transfer during the evolution of E. coli. Analysis of the status of some neonates further suggests that neonatal meningitis results from a balance between bacterial genes of virulence and host factors.