3D printing of silver-doped polycaprolactone-poly(propylene succinate) composite scaffolds for skin tissue engineering
3D printing of silver-doped polycaprolactone-poly(propylene succinate) composite scaffolds for skin tissue engineering
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DOI：
10.1088/1748-605x/ab7417
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发表时间：
2020-03-01
影响因子：
4
通讯作者：
Koc, Bahattin
中科院分区：
文献类型：
--
作者：
Afghah, Ferdows;Ullah, Mohib;Koc, Bahattin
基于支架的组织工程方法已被广泛用于疾病或创伤引起的皮肤再生或伤口愈合。对于理想的完整愈合过程，支架结构需要满足生物相容性、生物降解性和抗菌性能的标准，以及为受损组织的再生提供几何必需品。本文介绍了一种基于聚己内酯-嵌段-聚(1，3-丙二酸丙二酸酯)(PCL-PPSU)的三维(3D)可打印共聚物的设计、合成和表征。与纯净的PCL相比，PCL-PPSU共聚物的3D打印提供了更低的加工温度，因此可以在所开发的共聚物中加入温度敏感型生物活性物质。此外，3D打印嵌段共聚物表现出增强的水解性和酶降解性。利用人真皮成纤维细胞(HDF)对所开发的共聚物进行了细胞活性和细胞毒性评价。在聚合物基质中添加硝酸银，可显著降低不同类型微生物在支架上的黏附力，而不会对体外培养的HDF细胞产生任何细胞毒性。结果表明，含有抗菌银颗粒的3D打印PCL-PPSU支架具有对3D打印技术的适应性、低加工温度、增强的降解行为和抗菌性能，有望成为一种新兴的皮肤再生治疗的生物材料。
Scaffold-based tissue engineering approaches have been commonly used for skin regeneration or wound healings caused by diseases or trauma. For an ideal complete healing process, scaffold structures need to meet the criteria of biocompatibility, biodegradability, and antimicrobial properties, as well as to provide geometrical necessities for the regeneration of damaged tissue. In this study, design, synthesis and characterization of a three dimensional (3D) printable copolymer based on polycaprolactone-block-poly(1,3-propylene succinate) (PCL-PPSu) including anti-microbial silver particles is presented. 3D printing of PCL-PPSu copolymers provided a lower processing temperature compared to neat PCL, hence, inclusion of temperature-sensitive bioactive reagents into the developed copolymer could be realized. In addition, 3D printed block copolymer showed an enhanced hydrolytic and enzymatic degradation behavior. Cell viability and cytotoxicity of the developed copolymer were evaluated by using human dermal fibroblast (HDF) cells. The addition of silver nitrate within the polymer matrix resulted in a significant decrease in the adhesion of different types of microorganisms on the scaffold without inducing any cytotoxicity on HDF cells in vitro. The results suggested that 3D printed PCL-PPSu scaffolds containing anti-microbial silver particles could be considered as a promising biomaterial for emerging skin regenerative therapies, in the light of its adaptability to 3D printing technology, low-processing temperature, enhanced degradation behavior and antimicrobial properties.