SYMPATHETIC NERVOUS ACTIVITY TO BROWN ADIPOSE-TISSUE INCREASES IN COLD-TOLERANT MICE
SYMPATHETIC NERVOUS ACTIVITY TO BROWN ADIPOSE-TISSUE INCREASES IN COLD-TOLERANT MICE
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DOI：
10.1016/0031-9384(94)90080-9
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发表时间：
1994-05-01
影响因子：
2.9
通讯作者：
ENGEL, BT
中科院分区：
文献类型：
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作者：
KAWATE, R;TALAN, MI;ENGEL, BT
棕色脂肪组织（BAT）被认为是负责增加产热冷驯化啮齿动物。我们测量了冷适应C57 BL/6 J小鼠（ACCLI）在冷刺激期间肩胛间BAT（IBAT）的交感神经活动（SNA）。通过每隔一周重复暴露于冷应激持续3周来实现冷驯化（耐冷性增加）。我们将这些动物的SNA与先前没有冷应激经历的小鼠（幼稚）的SNA进行了比较。在试验过程中，小鼠用乌拉坦和异氟烷麻醉，并用维库溴铵麻痹。交感神经活动直接记录从一个细神经IBAT。动物的身体尾部的骨盆区域覆盖有一个塑料袋，其中含有浆状的冰水，以降低结肠温度7摄氏度以下的控制水平，这需要大约20分钟。肩胛间的BAT-SNA增加在两组的冷应激，但ACCLI小鼠有较高的IBAT-SNA在冷应激比幼稚小鼠。这些发现证实了这一假设，即在急性冷暴露期间，冷适应小鼠有更大的交感神经流出到BAT脂肪细胞。
Brown adipose tissue (BAT) is thought to be responsible for increased heat production in cold-acclimated rodents. We measured sympathetic nerve activity (SNA) in interscapular BAT (IBAT) during cold stimulation in cold-acclimated C57BL/6J mice (ACCLI). Cold acclimation was achieved (cold tolerance was increased) by repeated exposure to cold stress every other week for 3 weeks. We compared SNA in these animals with SNA in mice that had no previous cold stress experience (naive). During the test, mice were anesthetized by urethane and isoflurane and were paralyzed with vecuronium bromide. Sympathetic nerve activity was recorded directly from one of the fine nerves to IBAT. The animal's body caudal to the pelvic area was covered with a plastic bag containing a slurry of ice water to decrease colonic temperature 7 degrees C below control level, which took approximately 20 min. Interscapular BAT-SNA increased during cold stress in both groups, but ACCLI mice had higher IBAT-SNA during cold stress than naive mice. These findings confirmed the hypothesis that during the acute cold exposure, cold-acclimated mice have greater sympathetic outflow to BAT adipocytes.