Studies on in vivo priming of the TNP-reactive cytotoxic effector cell system. I. Comparison of the effects of intravenous inoculation with TNP-conjugated cells on the development of contact sensitivity and cell-mediated lympholysis.
Studies on in vivo priming of the TNP-reactive cytotoxic effector cell system. I. Comparison of the effects of intravenous inoculation with TNP-conjugated cells on the development of contact sensitivity and cell-mediated lympholysis.
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TNP 反应性细胞毒性效应细胞系统体内引发的研究。
DOI：
10.4049/jimmunol.123.1.423
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发表时间：
1979
影响因子：
4.4
通讯作者：
W. Terry
中科院分区：
文献类型：
--
作者：
H. Fujiwara;R. Levy;G. Shearer;W. Terry
对静脉注射的效果进行了比较。接种三硝基苯基 (TNP) 缀合的同基因细胞对随后体外产生 TNP 反应性效应细胞活性和体内 TNP 接触敏感性的发展。在 2,4,6-三硝基-1-氯苯 (TNCB) 涂漆之前给予同源 TNP 结合的脾细胞会消除动物产生 TNP 接触敏感性的能力。相比之下，通过随后用 TNP 缀合细胞进行体外致敏来测量，相同的处理导致 TNP 反应性细胞毒性效应细胞活性的产生明显增强。讨论了在与诱导 TNP 接触敏感性无反应的条件相同的条件下引发 TNP 反应性细胞毒性效应细胞活性增强的可能机制。
A comparison was made of the effects of i.v. inoculation of trinitrophenyl-(TNP) conjugated syngeneic cells on the subsequent in vitro generation of TNP-reactive effector cell activity and on the in vivo development of TNP-contact sensitivity. The administration of syngeneic TNP-conjugated spleen cells before 2,4,6-trinitro-1-chlorobenzene (TNCB) painting abolished the capability of animals to develop TNP-contact sensitivity. In contrast, the same treatment resulted in an appreciable augmentation in the generation of TNP-reactive cytotoxic effector cell activity as measured by subsequent in vitro sensitization with TNP-conjugated cells. The possible mechanisms by which enhanced TNP-reactive cytotoxic effector cell activity was elicited under conditions identical to those that induced unresponsiveness for TNP-contact sensitivity are discussed.