Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells
Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells
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DOI：
10.1007/s00299-006-0238-y
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发表时间：
2007-02-01
影响因子：
6.2
通讯作者：
Xia, Gui-Xian
中科院分区：
文献类型：
--
作者：
Chen, An-Ping;Wang, Gui-Ling;Xia, Gui-Xian
盐生植物 Thellungiella halophila（盐水芹）是研究植物耐盐分子机制的理想模型系统。在此，我们报告了使用裂殖酵母作为功能系统从 T. halophila 中鉴定出应激反应性亲环蛋白基因 (ThCYP1)。 ThCYP1 的表达可由盐、脱落酸 (ABA)、H2O2 和热休克高度诱导。 ThCYP1 基因的异位过度表达增强了裂殖酵母和烟草 (Nicotiana tabacum L.) cv. 的耐盐能力。亮黄 2 (BY-2) 细胞显着。 ThCYP1在根、茎、叶和花中组成型表达，其中在根和花中表达量较高。 ThCYP1 蛋白在细胞内广泛分布，但在细胞核中显着富集。目前的结果表明，ThCYP1 可能通过调节某些应激相关蛋白的适当折叠或参与信号转导过程来参与盐芥的应激反应。
The halophyte Thellungiella halophila (salt cress) is an ideal model system for studying the molecular mechanisms of salinity tolerance in plants. Herein, we report the identification of a stress-responsive cyclophilin gene (ThCYP1) from T. halophila, using fission yeast as a functional system. The expression of ThCYP1 is highly inducible by salt, abscisic acid (ABA), H2O2 and heat shock. Ectopic overexpression of the ThCYP1 gene enhance the salt tolerance capacity of fission yeast and tobacco (Nicotiana tabacum L.) cv. Bright Yellow 2 (BY-2) cells significantly. ThCYP1 is expressed constitutively in roots, stems, leaves and flowers, with higher expression occurring in the roots and flowers. The ThCYP1 proteins are distributed widely within the cell, but are enriched significantly in the nucleus. The present results suggest that ThCYP1 may participate in response to stresses in the salt cress, perhaps by regulating appropriate folding of certain stress-related proteins, or in the signal transduction processes.